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Literature summary for 3.5.4.3 extracted from

  • Yuan, G.; Bin, J.C.; McKay, D.J.; Snyder, F.F.
    Cloning and characterization of human guanine deaminase. Purification and partial amino acid sequence of the mouse protein (1999), J. Biol. Chem., 274, 8175-8180.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme has been implicated in cancer, liver disease, saturine gout, psoriasis and autoimmune disease, histochemical method for demonstration of guanase should be useful clinically, guanase activity increases significantly in lung and gastric cancer tissues or in patients with liver diseases like hepatitis, guanase is a biochemical indicator of rejection in liver transplant recipients, serum enzyme activity is the most sensitive indicator of liver disease, inhibition of guanase has beneficial implications in cancer chemotherapy, an inhibitor as chemotherapeutic agent selectively inhibit the growth of rapidly proliferating cancerous cells via depletion of the purine nucleotide pool Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloned from kidney and brain cDNA libraries, PCR, subcloned into bacterial expression vector pMAL-c2, expressed in mice DH5-alpha cells and purified Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0095
-
guanine recombinant enzyme, expressed in DH5-alpha cells Homo sapiens
0.011
-
guanine
-
Sus sp.
0.0153
-
guanine
-
Homo sapiens
0.0227
-
guanine
-
Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ addition results in 2fold increase of activity Homo sapiens
Zn2+ association of 1 atom per enzyme monomer Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Mus musculus
50000
-
2 * 50000, SDS-PAGE Sus sp.
50000
-
2 * 50000, SDS-PAGE Homo sapiens
50000
-
4 * 50000, erythrocytic enzyme, 5% of the protein present, SDS-PAGE Mus musculus
51040
-
calculated from cDNA corresponding to an open reading frame Homo sapiens
55000
-
2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups Sus sp.
55000
-
2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups Homo sapiens
59000
-
2 * 59000, SDS-PAGE Homo sapiens
100000
-
gel filtration Homo sapiens
200000
-
-
Homo sapiens
200000
-
erythrocytic enzyme, 5% of the protein present Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + H2O Mus musculus
-
xanthine + NH3
-
ir
guanine + H2O Sus sp.
-
xanthine + NH3
-
ir
guanine + H2O Homo sapiens
-
xanthine + NH3
-
ir

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y2T3 human
-
Mus musculus
-
mouse
-
Sus sp.
-
pig
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
brain
-
Sus sp.
-
brain
-
Homo sapiens
-
erythrocyte
-
Mus musculus
-
intestine small intestine Mus musculus
-
kidney
-
Homo sapiens
-
liver
-
Mus musculus
-
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.53
-
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + H2O
-
Mus musculus xanthine + NH3
-
ir
guanine + H2O
-
Sus sp. xanthine + NH3
-
ir
guanine + H2O
-
Homo sapiens xanthine + NH3
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Mus musculus
dimer 2 * 50000, SDS-PAGE Sus sp.
dimer 2 * 50000, SDS-PAGE Homo sapiens
dimer 2 * 59000, SDS-PAGE Homo sapiens
dimer 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups Sus sp.
dimer 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups Homo sapiens
tetramer 4 * 50000, erythrocytic enzyme, 5% of the protein present, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
human guanine deaminase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant protein, expressed in mice DH5-alpha cells, Km remains constant between pH 6.5 and pH 7.5 Homo sapiens