Application | Comment | Organism |
---|---|---|
medicine | enzyme has been implicated in cancer, liver disease, saturine gout, psoriasis and autoimmune disease, histochemical method for demonstration of guanase should be useful clinically, guanase activity increases significantly in lung and gastric cancer tissues or in patients with liver diseases like hepatitis, guanase is a biochemical indicator of rejection in liver transplant recipients, serum enzyme activity is the most sensitive indicator of liver disease, inhibition of guanase has beneficial implications in cancer chemotherapy, an inhibitor as chemotherapeutic agent selectively inhibit the growth of rapidly proliferating cancerous cells via depletion of the purine nucleotide pool | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cloned from kidney and brain cDNA libraries, PCR, subcloned into bacterial expression vector pMAL-c2, expressed in mice DH5-alpha cells and purified | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxymercuribenzoate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
guanine | recombinant enzyme, expressed in DH5-alpha cells | Homo sapiens | |
0.011 | - |
guanine | - |
Sus sp. | |
0.0153 | - |
guanine | - |
Homo sapiens | |
0.0227 | - |
guanine | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | addition results in 2fold increase of activity | Homo sapiens | |
Zn2+ | association of 1 atom per enzyme monomer | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Mus musculus |
50000 | - |
2 * 50000, SDS-PAGE | Sus sp. |
50000 | - |
2 * 50000, SDS-PAGE | Homo sapiens |
50000 | - |
4 * 50000, erythrocytic enzyme, 5% of the protein present, SDS-PAGE | Mus musculus |
51040 | - |
calculated from cDNA corresponding to an open reading frame | Homo sapiens |
55000 | - |
2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups | Sus sp. |
55000 | - |
2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups | Homo sapiens |
59000 | - |
2 * 59000, SDS-PAGE | Homo sapiens |
100000 | - |
gel filtration | Homo sapiens |
200000 | - |
- |
Homo sapiens |
200000 | - |
erythrocytic enzyme, 5% of the protein present | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | Mus musculus | - |
xanthine + NH3 | - |
ir | |
guanine + H2O | Sus sp. | - |
xanthine + NH3 | - |
ir | |
guanine + H2O | Homo sapiens | - |
xanthine + NH3 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y2T3 | human | - |
Mus musculus | - |
mouse | - |
Sus sp. | - |
pig | - |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Sus sp. | - |
brain | - |
Homo sapiens | - |
erythrocyte | - |
Mus musculus | - |
intestine | small intestine | Mus musculus | - |
kidney | - |
Homo sapiens | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.53 | - |
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | - |
Mus musculus | xanthine + NH3 | - |
ir | |
guanine + H2O | - |
Sus sp. | xanthine + NH3 | - |
ir | |
guanine + H2O | - |
Homo sapiens | xanthine + NH3 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Mus musculus |
dimer | 2 * 50000, SDS-PAGE | Sus sp. |
dimer | 2 * 50000, SDS-PAGE | Homo sapiens |
dimer | 2 * 59000, SDS-PAGE | Homo sapiens |
dimer | 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups | Sus sp. |
dimer | 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups | Homo sapiens |
tetramer | 4 * 50000, erythrocytic enzyme, 5% of the protein present, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
human guanine deaminase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant protein, expressed in mice DH5-alpha cells, Km remains constant between pH 6.5 and pH 7.5 | Homo sapiens |