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Literature summary for 3.5.4.3 extracted from
- Guanine deaminase from rabbit liver (1978), Methods Enzymol., 51, 512-517.
General Stability
| General Stability | Organism |
|---|---|
| stabilized by 2-mercaptoethanol, labile to heat | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-aminoimidazole-4-carboxamide | - |
Oryctolagus cuniculus |  |
| 5-aminoimidazole-4-carboxamide ribonucleoside | - |
Oryctolagus cuniculus | |
| iodoacetic acid | - |
Oryctolagus cuniculus | |
| p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus | |
| tetrahydrofolic acid | competitive inhibitor | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0125 | - |
guanine | at pH 7 | Oryctolagus cuniculus | |
| 0.333 | - |
8-Azaguanine | at pH 6 | Oryctolagus cuniculus | |
| 0.8 | - |
6-thioguanine | - |
Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + H2O | Oryctolagus cuniculus | - |
xanthine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
rabbit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.2 | - |
liver enzyme | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-thioguanine + H2O | - |
Oryctolagus cuniculus | 6-thioxanthine + NH3 | - |
? | |
| 8-azaguanine + H2O | - |
Oryctolagus cuniculus | 8-azaxanthine + NH3 | - |
? | |
| guanine + H2O | - |
Oryctolagus cuniculus | xanthine + NH3 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
relatively stable up to 50°C | Oryctolagus cuniculus |
| 55 | - |
loss of catalytic activity upon incubation for 20 min | Oryctolagus cuniculus |
| 60 | - |
30 min incubation, liver enzyme completely inactivated | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
8-azaguanine as substrate | Oryctolagus cuniculus |
| 6.8 | - |
pH optimum is independent of the buffer system | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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