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Literature summary for 3.5.4.3 extracted from
- Guanine deaminase from rat brain. Purification, characteristics, and contribution to ammoniagenesis in the brain (1982), J. Biochem., 91, 167-176.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | 0.4 mM, residual enzyme activity 112% | Rattus norvegicus |  |
| ATP | 0.4 mM, residual enzyme activity 104% | Rattus norvegicus | |
| uric acid | 0.4 mM, residual enzyme activity 105% | Rattus norvegicus | |
| xanthine | 0.4 mM, residual enzyme activity 110% | Rattus norvegicus | |
| XMP | 0.4 mM, residual enzyme activity 113% | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-aminoimidazole-4-carboxamide | - |
Rattus norvegicus | |
| 5-aminoimidazole-4-carboxamide ribonucleoside | AICAR | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
guanine | - |
Sus sp. | |
| 0.17 | - |
guanine | - |
Rattus norvegicus | |
| 0.67 | - |
8-Azaguanine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Sus sp. |
| 52000 | - |
2 * 52000, brain and liver enzymes, SDS-PAGE | Rattus norvegicus |
| 105000 | - |
gel filtration | Rattus norvegicus |
| 108000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + H2O | Rattus norvegicus | - |
xanthine + NH3 | - |
? | |
| guanine + H2O | Sus sp. | - |
xanthine + NH3 | - |
? | |
| guanine + H2O | Rattus norvegicus Wistar | - |
xanthine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
| Rattus norvegicus | - |
Wistar strain | - |
| Rattus norvegicus Wistar | - |
Wistar strain | - |
| Sus sp. | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| brain | - |
Sus sp. | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.7 | - |
purified brain enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 10 mM phosphate buffer, pH 7.0, 0.1 mM dithiothreitol, can be stored for more than 2 weeks without appreciable loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8-azaguanine + H2O | - |
Rattus norvegicus | 8-azaxanthine + NH3 | - |
? | |
| 8-azaguanine + H2O | - |
Rattus norvegicus Wistar | 8-azaxanthine + NH3 | - |
? | |
| guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Sus sp. | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Rattus norvegicus Wistar | xanthine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Sus sp. |
| dimer | 2 * 52000, brain and liver enzymes, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9.4 | - |
Rattus norvegicus |
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