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Literature summary for 3.5.4.3 extracted from
- Studies of rabbit brain, intestine and liver guanine aminohydrolase (1984), Comp. Biochem. Physiol. B, 77, 619-627.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme has been implicated in cancer, liver disease, saturine gout, psoriasis and autoimmune disease, histochemical method for demonstration of guanase should be useful clinically, guanase activity increases significantly in lung and gastric cancer tissues or in patients with liver diseases like hepatitis, guanase is a biochemical indicator of rejection in liver transplant recipients, serum enzyme activity is the most sensitive indicator of liver disease, inhibition of guanase has beneficial implications in cancer chemotherapy, an inhibitor as chemotherapeutic agent selectively inhibit the growth of rapidly proliferating cancerous cells via depletion of the purine nucleotide pool | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-aminoimidazole-4-carboxamide | - |
Homo sapiens |  |
| 5-aminoimidazole-4-carboxamide | - |
Oryctolagus cuniculus | |
| F- | - |
Homo sapiens | |
| F- | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00296 | - |
guanine | brain enzyme at pH 5.5 | Oryctolagus cuniculus | |
| 0.0033 | - |
guanine | liver enzyme at pH 9.0 | Oryctolagus cuniculus | |
| 0.0035 | - |
guanine | brain enzyme at pH 9.0 | Oryctolagus cuniculus | |
| 0.0036 | - |
guanine | intestine enzyme at pH 9.0 | Oryctolagus cuniculus | |
| 0.0038 | - |
guanine | at pH 7 , intestine enzyme at pH 5.5 | Oryctolagus cuniculus | |
| 0.00451 | - |
guanine | intestine enzyme at pH 5.5 | Oryctolagus cuniculus | |
| 0.00476 | - |
guanine | liver enzyme at pH 5.1 | Oryctolagus cuniculus | |
| 0.00478 | - |
guanine | intestine enzyme at pH 5.1 | Oryctolagus cuniculus | |
| 0.00481 | - |
guanine | brain enzyme at pH 5.1, liver enzyme at pH 7.0 | Oryctolagus cuniculus | |
| 0.00509 | - |
guanine | intestine enzyme at pH 7.0 | Oryctolagus cuniculus | |
| 0.00518 | - |
guanine | liver enzyme at pH 8.5 | Oryctolagus cuniculus | |
| 0.00527 | - |
guanine | liver enzyme at pH 6.0 | Oryctolagus cuniculus | |
| 0.00546 | - |
guanine | brain enzyme at pH 8.5 | Oryctolagus cuniculus | |
| 0.0056 | - |
guanine | - |
Homo sapiens | |
| 0.0056 | - |
guanine | at pH 8 | Oryctolagus cuniculus | |
| 0.0058 | - |
guanine | liver enzyme at pH 8.0 | Oryctolagus cuniculus | |
| 0.00586 | - |
guanine | brain enzyme at pH 6.0 | Oryctolagus cuniculus | |
| 0.0061 | - |
guanine | brain and intestine enzymes at pH 8 | Oryctolagus cuniculus | |
| 0.00611 | - |
guanine | brain and intestine enzymes at pH 8 | Oryctolagus cuniculus | |
| 0.0063 | - |
guanine | brain enzyme at pH 7.0 | Oryctolagus cuniculus | |
| 0.00671 | - |
guanine | intestine enzyme at pH 8.5 | Oryctolagus cuniculus | |
| 0.0068 | - |
guanine | at pH 7.4 | Oryctolagus cuniculus | |
| 0.00707 | - |
guanine | intestine enzyme at pH 7.5 | Oryctolagus cuniculus | |
| 0.00726 | - |
guanine | brain enzyme at pH 7.5 | Oryctolagus cuniculus | |
| 0.0073 | - |
guanine | intestine enzyme at pH 6.5 | Oryctolagus cuniculus | |
| 0.00736 | - |
guanine | liver enzyme at pH 6.5 | Oryctolagus cuniculus | |
| 0.00747 | - |
guanine | intestine enzyme at pH 6.0 | Oryctolagus cuniculus | |
| 0.00762 | - |
guanine | liver enzyme at pH 7.5 | Oryctolagus cuniculus | |
| 0.00819 | - |
guanine | brain enzyme at pH 6.5 | Oryctolagus cuniculus | |
| 0.0105 | - |
guanine | at pH 8.2 | Oryctolagus cuniculus | |
| 0.0125 | - |
guanine | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, SDS-PAGE | Bos taurus |
| 49500 | - |
2 * 49500, SDS-PAGE | Oryctolagus cuniculus |
| 50000 | - |
2 * 50000, SDS-PAGE | Homo sapiens |
| 50000 | - |
2 * 50000, SDS-PAGE | Oryctolagus cuniculus |
| 50000 | - |
2 * 50000, SDS-PAGE | Sus sp. |
| 52000 | - |
2 * 52000, brain and liver enzymes, SDS-PAGE | Bos taurus |
| 52000 | - |
2 * 52000, brain and liver enzymes, SDS-PAGE | Oryctolagus cuniculus |
| 56000 | - |
liver enzyme, gel filtration | Oryctolagus cuniculus |
| 59000 | - |
2 * 59000, SDS-PAGE | Homo sapiens |
| 92000 | - |
- |
Oryctolagus cuniculus |
| 92200 | - |
gel filtration, non denaturing PAGE | Oryctolagus cuniculus |
| 95000 | - |
- |
Bos taurus |
| 95800 | - |
liver enzyme, gel filtration | Oryctolagus cuniculus |
| 100000 | - |
- |
Homo sapiens |
| 100000 | - |
- |
Oryctolagus cuniculus |
| 105000 | - |
- |
Bos taurus |
| 107800 | - |
crosslinking | Oryctolagus cuniculus |
| 110000 | - |
- |
Sus sp. |
| 110000 | - |
liver, gel filtration | Homo sapiens |
| 110000 | - |
liver, gel filtration | Oryctolagus cuniculus |
| 120000 | - |
- |
Homo sapiens |
| 120000 | - |
brain and liver enzymes | Cavia porcellus |
| 200000 | - |
brain enzyme | Homo sapiens |
| 240000 | - |
gel filtration without thiol present, enzyme aggregation | Homo sapiens |
| 240000 | - |
gel filtration without thiol present, enzyme aggregation | Sus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + H2O | Cavia porcellus | - |
xanthine + NH3 | - |
? | |
| guanine + H2O | Homo sapiens | - |
xanthine + NH3 | - |
? | |
| guanine + H2O | Bos taurus | - |
xanthine + NH3 | - |
? | |
| guanine + H2O | Oryctolagus cuniculus | - |
xanthine + NH3 | - |
? | |
| guanine + H2O | Ovis ammon | - |
xanthine + NH3 | - |
? | |
| guanine + H2O | Sus sp. | - |
xanthine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
cow | - |
| Cavia porcellus | - |
guinea pig | - |
| Homo sapiens | - |
human | - |
| Oryctolagus cuniculus | - |
rabbit | - |
| Ovis ammon | - |
sheep | - |
| Sus sp. | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Cavia porcellus | - |
| brain | - |
Homo sapiens | - |
| brain | - |
Oryctolagus cuniculus | - |
| brain | - |
Sus sp. | - |
| intestine | - |
Oryctolagus cuniculus | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| pancreas | - |
Homo sapiens | - |
| pancreas | - |
Oryctolagus cuniculus | - |
| spleen | - |
Homo sapiens | - |
| spleen | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.2 | - |
intestine enzyme, purification at 0-4°C | Oryctolagus cuniculus |
| 16.8 | - |
brain enzyme, purification at room temperature | Oryctolagus cuniculus |
| 17.2 | - |
liver enzyme, purification at room temperature | Oryctolagus cuniculus |
| 19.3 | - |
liver enzyme, purification at 0-4°C | Oryctolagus cuniculus |
| 22.4 | - |
brain enzyme, purification at 0-4°C | Oryctolagus cuniculus |
| 25.4 | - |
intestine enzyme, purification at room temperature | Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified enzyme stabilized by storage in 30-40% glycerol | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + H2O | - |
Cavia porcellus | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Homo sapiens | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Bos taurus | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Oryctolagus cuniculus | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Ovis ammon | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Sus sp. | xanthine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Homo sapiens |
| dimer | 2 * 50000, SDS-PAGE | Oryctolagus cuniculus |
| dimer | 2 * 50000, SDS-PAGE | Sus sp. |
| dimer | 2 * 59000, SDS-PAGE | Homo sapiens |
| dimer | 2 * 48000, SDS-PAGE | Bos taurus |
| dimer | 2 * 52000, brain and liver enzymes, SDS-PAGE | Bos taurus |
| dimer | 2 * 52000, brain and liver enzymes, SDS-PAGE | Oryctolagus cuniculus |
| dimer | 2 * 49500, SDS-PAGE | Oryctolagus cuniculus |
| monomer | 1 * 56000 | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Oryctolagus cuniculus |
| 7.7 | - |
- |
Oryctolagus cuniculus |
| 8 | - |
- |
Homo sapiens |
| 8 | - |
- |
Oryctolagus cuniculus |
| 8 | 8.5 | - |
Oryctolagus cuniculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | activity relatively low at pH 5.1-5.5, increase in activity above pH 6.0 until pH 8.5, above pH 8.5 activity starts to drop off | Oryctolagus cuniculus |
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