Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aminoimidazole-4-carboxamide | - |
Homo sapiens | |
5-aminoimidazole-4-carboxamide | - |
Oryctolagus cuniculus | |
5-aminoimidazole-4-carboxamide | - |
Rattus norvegicus | |
5-aminoimidazole-4-carboxamide ribonucleoside | - |
Oryctolagus cuniculus | |
5-aminoimidazole-4-carboxamide ribonucleoside | - |
Rattus norvegicus | |
iodoacetamide | 2 mM mildly inhibitory | Oryctolagus cuniculus | |
iodoacetic acid | 0.1 mM 7% loss in the activity, 1 mM 29% loss in the activity after 30 min incubation | Homo sapiens | |
iodoacetic acid | - |
Oryctolagus cuniculus | |
p-hydroxymercuribenzoate | 0.0001 M 91% loss of activity after 30 min incubation | Homo sapiens | |
p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
tetrahydrofolic acid | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
guanine | - |
Oryctolagus cuniculus | |
0.0042 | - |
guanine | - |
Bos taurus | |
0.0056 | - |
guanine | - |
Homo sapiens | |
0.0056 | - |
guanine | - |
Oryctolagus cuniculus | |
0.011 | - |
guanine | - |
Sus sp. | |
0.0125 | - |
guanine | at pH 7 | Oryctolagus cuniculus | |
0.0153 | - |
guanine | at pH 6 | Homo sapiens | |
0.0156 | - |
guanine | at pH 8.0 | Homo sapiens | |
0.0166 | - |
guanine | at pH 7.0 | Homo sapiens | |
0.0178 | - |
guanine | at pH 7.5 | Homo sapiens | |
0.0182 | - |
guanine | - |
Oryctolagus cuniculus | |
0.0182 | - |
guanine | at pH 6 | Homo sapiens | |
0.02 | - |
guanine | at pH 6.5 | Homo sapiens | |
0.0313 | - |
guanine | at pH 5.5 | Homo sapiens | |
0.037 | - |
guanine | at pH 9.0 | Homo sapiens | |
0.0417 | - |
guanine | at pH 8.5 | Homo sapiens | |
0.105 | - |
guanine | - |
Oryctolagus cuniculus | |
0.17 | - |
guanine | - |
Rattus norvegicus | |
0.2 | - |
8-Azaguanine | at pH 6 | Homo sapiens | |
0.23 | - |
8-Azaguanine | - |
Oryctolagus cuniculus | |
0.33 | - |
8-Azaguanine | - |
Sus sp. | |
0.333 | - |
8-Azaguanine | at pH 6 | Oryctolagus cuniculus | |
0.67 | - |
8-Azaguanine | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Homo sapiens |
50000 | - |
2 * 50000, SDS-PAGE | Oryctolagus cuniculus |
50000 | - |
2 * 50000, SDS-PAGE | Sus sp. |
52000 | - |
2 * 52000, brain and liver enzymes, SDS-PAGE | Rattus norvegicus |
52000 | - |
2 * 52000, brain and liver enzymes, SDS-PAGE | Bos taurus |
52000 | - |
2 * 52000, brain and liver enzymes, SDS-PAGE | Oryctolagus cuniculus |
55000 | - |
- |
Homo sapiens |
55000 | - |
liver, single polypeptide chain, gel filtration | Rattus norvegicus |
55000 | - |
liver, single polypeptide chain, gel filtration | Oryctolagus cuniculus |
55000 | - |
2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups | Sus sp. |
59000 | - |
- |
Oryctolagus cuniculus |
59000 | - |
2 * 59000, SDS-PAGE | Homo sapiens |
95000 | - |
- |
Bos taurus |
100000 | - |
- |
Homo sapiens |
100000 | - |
2 forms of liver enzyme | Oryctolagus cuniculus |
105000 | - |
- |
Rattus norvegicus |
105000 | - |
- |
Bos taurus |
110000 | - |
- |
Rattus norvegicus |
110000 | - |
- |
Sus sp. |
110000 | - |
liver, gel filtration | Homo sapiens |
110000 | - |
liver, gel filtration | Oryctolagus cuniculus |
120000 | - |
gel filtration | Homo sapiens |
170000 | - |
2 forms of liver enzyme | Oryctolagus cuniculus |
200000 | - |
- |
Homo sapiens |
207000 | - |
2 forms of liver enzyme | Oryctolagus cuniculus |
525000 | - |
2 forms of liver enzyme | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | Homo sapiens | - |
xanthine + NH3 | - |
? | |
guanine + H2O | Rattus norvegicus | - |
xanthine + NH3 | - |
? | |
guanine + H2O | Bos taurus | - |
xanthine + NH3 | - |
? | |
guanine + H2O | Oryctolagus cuniculus | - |
xanthine + NH3 | - |
? | |
guanine + H2O | Sus sp. | - |
xanthine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
cow | - |
Homo sapiens | - |
human | - |
Oryctolagus cuniculus | - |
rabbit | - |
Rattus norvegicus | - |
rat | - |
Sus sp. | - |
pig | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Bos taurus |
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.5 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-thioguanine + H2O | - |
Oryctolagus cuniculus | 6-thioxanthine + NH3 | - |
? | |
6-thioguanine + H2O | - |
Sus sp. | 6-thioxanthine + NH3 | - |
? | |
8-azaguanine + H2O | - |
Homo sapiens | 8-azaxanthine + NH3 | - |
? | |
8-azaguanine + H2O | - |
Rattus norvegicus | 8-azaxanthine + NH3 | - |
? | |
8-azaguanine + H2O | - |
Oryctolagus cuniculus | 8-azaxanthine + NH3 | - |
? | |
8-azaguanine + H2O | - |
Sus sp. | 8-azaxanthine + NH3 | - |
? | |
guanine + H2O | - |
Homo sapiens | xanthine + NH3 | - |
? | |
guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
? | |
guanine + H2O | - |
Bos taurus | xanthine + NH3 | - |
? | |
guanine + H2O | - |
Oryctolagus cuniculus | xanthine + NH3 | - |
? | |
guanine + H2O | - |
Sus sp. | xanthine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Homo sapiens |
dimer | 2 * 50000, SDS-PAGE | Oryctolagus cuniculus |
dimer | 2 * 50000, SDS-PAGE | Sus sp. |
dimer | 2 * 59000, SDS-PAGE | Homo sapiens |
dimer | 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups | Homo sapiens |
dimer | 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups | Oryctolagus cuniculus |
dimer | 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups | Sus sp. |
dimer | 2 * 52000, brain and liver enzymes, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 52000, brain and liver enzymes, SDS-PAGE | Bos taurus |
dimer | 2 * 52000, brain and liver enzymes, SDS-PAGE | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Oryctolagus cuniculus |
40 | - |
stable up to | Homo sapiens |
45 | - |
relatively stable, incubation for 5 min, 3% loss in activity, 17% after 30 min | Homo sapiens |
55 | - |
- |
Oryctolagus cuniculus |
55 | - |
becomes labile at, loses 21% of activity after 5 min incubation, 49% after 30 min | Homo sapiens |
65 | - |
activity completely lost after 30 min incubation | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
liver enzyme | Oryctolagus cuniculus |
6 | - |
8-azaguanine as substrate | Homo sapiens |
6 | - |
8-azaguanine as substrate | Oryctolagus cuniculus |
6.8 | - |
- |
Oryctolagus cuniculus |
8 | - |
- |
Homo sapiens |
8 | - |
- |
Rattus norvegicus |
8 | - |
- |
Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Homo sapiens |