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Literature summary for 3.5.4.27 extracted from

  • te Boemmelstroet, B.W.; Hensgens, C.M.H.; Geerts, W.J.; Keltjens, J.T.; van der Drift, C.; Vogels, G.D.
    Purification and properties of 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanosarcina barkeri (1990), J. Bacteriol., 172, 564-571.
    View publication on PubMedView publication on EuropePMC
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General Stability

General Stability Organism
detergents are essential for stability Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 9-18 mM: no stimulation Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, SDS-PAGE Methanosarcina barkeri
82000
-
 non-denaturing PAGE Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Oxidation Stability

Oxidation Stability Organism
stable to oxygen Methanosarcina barkeri
the purified enzyme is more resistant to inactivation by O2 than the enzyme in cell extract Methanosarcina barkeri

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
470
-
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanosarcina barkeri N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Methanosarcina barkeri