Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | competitive | Escherichia coli | |
phosphate | partial uncompetitive at low concentration, competitive at concentrations above 6 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, cooperative reaction with a Hill coefficient of 1.3 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + 3 H2O | Escherichia coli | first committed step in the biosynthesis of riboflavin, overview | formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + 4 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + 2 phosphate | 2 C-N bonds are hydrolysed, releasing formate, with simultaneous removal of the terminal diphosphate, reaction mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + 3 H2O | - |
Escherichia coli | formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate | spontaneous isomerization into the alpha-isomer, also the product can easily spontaneously decompose under formation of 2,4,5-triamino-4-(3H)-pyrimidone | ? | |
GTP + 3 H2O | first committed step in the biosynthesis of riboflavin, overview | Escherichia coli | formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GTP-8-formylhydrolase | - |
Escherichia coli |
guanosine triphosphate cyclohydrolase II | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
diphosphate | pH 8.5, 37°C | Escherichia coli |