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Literature summary for 3.5.4.25 extracted from

  • Foor, F.; Brown, G.M.
    Purification and properties of guanosine triphosphate cyclohydrolase II from Escherichia coli (1975), J. Biol. Chem., 250, 3545-3551.
    View publication on PubMed
Search for more literature for 3.5.4.25

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 GTP
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cations Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli
-
 formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.223
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 1 mg/ml, pH8, in presence of EDTA and an excess of Mg2+, quite stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Escherichia coli formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.2 10.5 half of maximal activity Escherichia coli