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Literature summary for 3.5.4.23 extracted from
- Active center and mode of reaction of blasticidin S deaminase (1985), Agric. Biol. Chem., 49, 3355-3361.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | restores activity of pCMB treated enzyme up to 75% | Aspergillus terreus |  |
General Stability
| General Stability | Organism |
|---|---|
| unstable | Aspergillus terreus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | - |
Aspergillus terreus | |
| Rose bengal | 0.01%, enzyme loses more than 80% of its activity, photooxidation | Aspergillus terreus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
blasticidin S | - |
Aspergillus terreus | |
| 0.053 | - |
pyrimidinoblasticidin S | - |
Aspergillus terreus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| blasticidin S + H2O | Aspergillus terreus | - |
deaminohydroxyblasticidin S + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terreus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus terreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylblasticidin S + H2O | - |
Aspergillus terreus | acetyldeaminohydroxyblasticidin S + NH3 | - |
? | |
| blasticidin S + H2O | - |
Aspergillus terreus | deaminohydroxyblasticidin S + NH3 | - |
? | |
| cytomycin + H2O | - |
Aspergillus terreus | ? | - |
? | |
| additional information | cytosine, cytidine, deoxycytidine, CMP, d-CMP or purine nucleosides are no substrates | Aspergillus terreus | ? | - |
? | |
| pyrimidinoblasticidin S + H2O | - |
Aspergillus terreus | pyrimidinodeaminohydroxyblasticidin S + NH3 | - |
? | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | retains original activity | Aspergillus terreus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | inactivation below pH 4 for 1h, but stable below pH 7 | Aspergillus terreus |
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Release 2023.1 (January 2023)
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