Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.22 extracted from

  • Adams, E.
    Enzymes and intermediates of hydroxyproline degradation (1971), Methods Enzymol., 17, 266-306.
No PubMed abstract available
Search for more literature for 3.5.4.22

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 ultracentrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-1-pyrroline-4-hydroxy-2-carboxylate + H2O Pseudomonas putida
-
 2,5-dioxopentanoate + NH3
-
 ir

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.78
-
-
 Pseudomonas putida

Storage Stability

Storage Stability Organism
-15°C, fraction II, at least one month Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-1-pyrroline-4-hydroxy-2-carboxylate + H2O
-
 Pseudomonas putida 2,5-dioxopentanoate + NH3
-
 ir
L-1-pyrroline-4-hydroxy-2-carboxylate + H2O
-
 Pseudomonas putida 2,5-dioxopentanoate + NH3
-
 ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 fraction III, 5 min, slight loss in activity Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas putida