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Literature summary for 3.5.4.21 extracted from
- Purification and properties of creatinine iminohydrolase from Flavobacterium filamentosum (1985), J. Biol. Chem., 260, 3915-3922.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
5-fluorocytosine | - |
Flavobacterium filamentosum |  |
| 0.62 | - |
cytosine | - |
Flavobacterium filamentosum | |
| 5 | - |
creatinine | - |
Flavobacterium filamentosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Flavobacterium filamentosum | |
| Zn2+ | - |
Flavobacterium filamentosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44300 | - |
6 * 44300, SDS-PAGE | Flavobacterium filamentosum |
| 272500 | - |
gel filtration | Flavobacterium filamentosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatinine + H2O | Flavobacterium filamentosum | - |
N-methylhydantoin + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium filamentosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Flavobacterium filamentosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 39.8 | - |
- |
Flavobacterium filamentosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylcytosine + H2O | - |
Flavobacterium filamentosum | 3-methyluracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Flavobacterium filamentosum | 5-fluorouracil + NH3 | - |
? | |
| 5-methylcytosine + H2O | - |
Flavobacterium filamentosum | 5-methyluracil + NH3 | - |
? | |
| creatinine + H2O | - |
Flavobacterium filamentosum | N-methylhydantoin + NH3 | - |
? | |
| cytosine + H2O | - |
Flavobacterium filamentosum | uracil + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 44300, SDS-PAGE | Flavobacterium filamentosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Flavobacterium filamentosum |
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Release 2023.1 (January 2023)
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