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Literature summary for 3.5.4.16 extracted from

  • Maita, N.; Okada, K.; Hatakeyama, K.; Hakoshima, T.
    Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP (2002), Proc. Natl. Acad. Sci. USA, 99, 1212-1217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.16

Activating Compound

Activating Compound Comment Organism Structure
L-phenylalanine feed-forward stimulation at subsaturating concentration of GTP mediated by GTP cyclohydrolase I feedback regulatory protein, i.e. GFRP, in complex with the enzyme, binding site structure Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme complexed with recombinant wild-type and selenomethionine derivatized GFRP, the complex with the latter being used as CH3HGCl derivative, 5 mg/ml protein complex in 24% v/v 2-methyl-2,4-pentanediol, 75 mM Tris-HCl, pH 7.5, 50 mM KCl, 5 mM phenylalanine, X-ray diffraction structure determination and analysis at 2.7-2.8 A resolution, model building Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin i.e. BH4, feedback inhibition mediated by GTP cyclohydrolase I feedback regulatory protein, i.e. GFRP, in complex with the enzyme, binding site structure Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ essential, bound to 2 Cys and 1 His Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
GTP + H2O = formate + 7,8-dihydroneopterin 3'-triphosphate reaction and regulation mechanism, enzyme forms a stimulatory complex with the GTP cyclohydrolase I feedback regulatory protein, i.e. GFRP Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Rattus norvegicus formate + D-erythro-dihydroneopterin triphosphate
-
 ?
additional information enzyme is complexed with the GTP cyclohydrolase I feedback regulatory protein, i.e. GFRP, in a ratio of 1:2, complex formation is induced by phenylalanine and (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin Rattus norvegicus ?
-
 ?