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Literature summary for 3.5.4.10 extracted from
- The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping (1996), J. Biol. Chem., 271, 2225-2233.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | higher activity in buffers containing 50 mM | Homo sapiens |  |
| NaCl | higher activity in buffers containing 150 mM | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64425 | - |
2 * 64425, open reading frame, polypeptide of 591 amino acids, 81% similar to chicken IMPCHase, truncation mutant, IMPCHase activity located within the NH2-terminal 223 amino acids | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | Homo sapiens | de novo purine synthesis step 10 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P31939 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| near to homogeneity from Escherichia coli expression mutants | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatoma cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.8 | - |
extract from Escherichia coli expression mutants after several pufification steps | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | - |
Homo sapiens | IMP + H2O | - |
ir | |
| 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | de novo purine synthesis step 10 | Homo sapiens | ? | - |
? | |
| 5-formamidoimidazole-4-carboxamide | - |
Homo sapiens | hypoxanthine + H2O | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 64425, open reading frame, polypeptide of 591 amino acids, 81% similar to chicken IMPCHase, truncation mutant, IMPCHase activity located within the NH2-terminal 223 amino acids | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Homo sapiens |
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