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Literature summary for 3.5.3.6 extracted from

  • Li, Z.; Kulakova, L.; Li, L.; Galkin, A.; Zhao, Z.; Nash, T.E.; Mariano, P.S.; Herzberg, O.; Dunaway-Mariano, D.
    Mechanisms of catalysis and inhibition operative in the arginine deiminase from the human pathogen Giardia lamblia (2009), Bioorg. Chem., 37, 149-161.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star(DE3) cells Giardia intestinalis

Protein Variants

Protein Variants Comment Organism
C424A catalytically inactive Giardia intestinalis

Inhibitors

Inhibitors Comment Organism Structure
(2S)-2-amino-5-(N''-cyanocarbamimidamido)pentanoic acid
-
 Giardia intestinalis
agmatine very weak, mixed-type inhibitor Giardia intestinalis
additional information Nomega-cyano-L-arginine does not inhibit Giardia lamblia arginine deiminase Giardia intestinalis
S-nitroso-L-homocysteine active site directed, irreversible inhibitor Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 Nomega-amino-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
0.16
-
 L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
2.1
-
 L-arginine ethyl ester in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64189
-
 2 * 64189, calculated from amino acid sequence Giardia intestinalis
64190
-
 2 * 64190, ESI-MS analysis Giardia intestinalis
127000
-
 gel filtration Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis Q27657
-
-
Giardia intestinalis isolate WB, clone 1267 Q27657
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and butyl-Sepharose column chromatography Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Giardia intestinalis L-citrulline + NH3
-
 ?
L-arginine + H2O
-
 Giardia intestinalis isolate WB, clone 1267 L-citrulline + NH3
-
 ?
L-arginine ethyl ester + H2O
-
 Giardia intestinalis ?
-
 ?
L-arginine ethyl ester + H2O
-
 Giardia intestinalis isolate WB, clone 1267 ?
-
 ?
additional information does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate Giardia intestinalis ?
-
 ?
additional information does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate Giardia intestinalis isolate WB, clone 1267 ?
-
 ?
Nalpha-benzoyl-L-arginine + H2O
-
 Giardia intestinalis ?
-
 ?
Nalpha-benzoyl-L-arginine + H2O
-
 Giardia intestinalis isolate WB, clone 1267 ?
-
 ?
Nomega-amino-L-arginine + H2O
-
 Giardia intestinalis hydrazine + L-citrulline
-
 ?
Nomega-amino-L-arginine + H2O
-
 Giardia intestinalis isolate WB, clone 1267 hydrazine + L-citrulline
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 64189, calculated from amino acid sequence Giardia intestinalis
homodimer 2 * 64190, ESI-MS analysis Giardia intestinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.58
-
 Nomega-amino-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
2.3
-
 L-arginine ethyl ester in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
2.5
-
 Nalpha-benzoyl-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
2.6
-
 L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Giardia intestinalis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 the kcat value is maximal from pH 5.5 to pH 9.0 Giardia intestinalis
6 8 the kcat_Km value is maximal between pH 6.0 and 8.0 Giardia intestinalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
40
-
 S-nitroso-L-homocysteine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.29
-
 Nomega-amino-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
2.3
-
 Nalpha-benzoyl-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
16
-
 L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis