Application | Comment | Organism |
---|---|---|
medicine | the recombinant enzyme can effectively inhibit H22 tumor growth at a total dose of 5 U/mouse over a 2-week dosing period | Pseudomonas plecoglossicida |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 with an N-terminal His6-tag | Pseudomonas plecoglossicida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46500 | - |
deduced ADI protein sequence | Pseudomonas plecoglossicida |
49000 | - |
SDS-PAGE | Pseudomonas plecoglossicida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas plecoglossicida | A7LKB0 | - |
- |
Pseudomonas plecoglossicida CGMCC2039 | A7LKB0 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelated agarose gel column chromatography | Pseudomonas plecoglossicida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.76 | - |
at pH 6.0 and 37°C | Pseudomonas plecoglossicida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + H2O | - |
Pseudomonas plecoglossicida | citrulline + NH3 | - |
? | |
L-Arg + H2O | - |
Pseudomonas plecoglossicida CGMCC2039 | citrulline + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas plecoglossicida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | - |
Pseudomonas plecoglossicida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | about 20% relative activity at 5°C, about 35% relative activity at 20°C, about 20% relative activity at 5°C, about 98% relative activity at 53°C, about 40% relative activity at 50°C, about 10% relative activity at 60°C | Pseudomonas plecoglossicida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas plecoglossicida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | - |
Pseudomonas plecoglossicida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | about 25% relative activity at pH 4.0, about 50% relative activity at pH 5.0, about 25% relative activity at pH 7.0, less than 10% relative activity at pH 8.0 | Pseudomonas plecoglossicida |