Literature summary for 3.5.3.6 extracted from

Li, L.; Li, Z.; Wang, C.; Xu, D.; Mariano, P.S.; Guo, H.; Dunaway-Mariano, D.
The electrostatic driving force for nucleophilic catalysis in L-arginine deiminase: a combined experimental and theoretical study (2008), Biochemistry, 47, 4721-4732.

Search for more literature for 3.5.3.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli
expressed in Escherichia coli BL21(DE3) cells	Bacillus cereus
expressed in Escherichia coli BL21(DE3) cells	Burkholderia mallei
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
C265S/C336S	the mutant is catalytically active	Bacillus cereus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.06	-	L-Arg	at pH 7.0 and 25°C	Bacillus cereus
0.09	-	L-Arg	at pH 5.6 and 25°C	Burkholderia mallei
0.14	-	L-Arg	at pH 5.6 and 25°C	Pseudomonas aeruginosa
0.32	-	L-Arg	at pH 6.0 and 25°C	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45900	-	electrospray mass spectrometry	Escherichia coli
45900	-	calculated from amino acid sequence	Escherichia coli
45920	-	electrospray mass spectrometry	Burkholderia mallei
46050	-	calculated from amino acid sequence	Burkholderia mallei
46940	-	electrospray mass spectrometry	Bacillus cereus
46940	-	calculated from amino acid sequence	Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	-	-	-
Burkholderia mallei	-	-	-
Escherichia coli	-	-	-
Pseudomonas aeruginosa	P13981	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose column chromatography, butyl-Sepharose column chromatography, and ammonium sulfate precipitation	Escherichia coli
DEAE-cellulose column chromatography, butyl-Sepharose column chromatography, and ammonium sulfate precipitation	Burkholderia mallei
DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, and ammonium sulfate precipitation	Pseudomonas aeruginosa
DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, hydroxyapatite column chromatography, and ammonium sulfate precipitation	Bacillus cereus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Arg + H2O	-	Escherichia coli	citrulline + NH3	-	?
L-Arg + H2O	-	Bacillus cereus	citrulline + NH3	-	?
L-Arg + H2O	-	Burkholderia mallei	citrulline + NH3	-	?
L-Arg + H2O	-	Pseudomonas aeruginosa	citrulline + NH3	-	?

Synonyms

Synonyms	Comment	Organism
L-arginine deiminase	-	Escherichia coli
L-arginine deiminase	-	Bacillus cereus
L-arginine deiminase	-	Burkholderia mallei
L-arginine deiminase	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.3	-	L-Arg	at pH 5.6 and 25°C	Burkholderia mallei
3.2	-	L-Arg	at pH 6.0 and 25°C	Escherichia coli
3.2	-	L-Arg	at pH 7.0 and 25°C	Bacillus cereus
6.3	-	L-Arg	at pH 5.6 and 25°C	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.4	7.8	-	Bacillus cereus
5.6	-	-	Burkholderia mallei
5.6	-	-	Pseudomonas aeruginosa
6	-	-	Escherichia coli

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Release 2023.1 (January 2023)