Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C406A | inactive mutant enzyme | Pseudomonas aeruginosa |
C406A | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
C406S | inactive mutant enzyme | Pseudomonas aeruginosa |
C406S | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
D166A | inactive mutant enzyme | Pseudomonas aeruginosa |
D166A | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
D280A | inactive mutant enzyme | Pseudomonas aeruginosa |
D280A | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
E224A | kcat/Km is 23684fold higher than the wild-type value | Pseudomonas aeruginosa |
E224A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
E224D | kcat/Km is 50000fold higher than the wild-type value | Pseudomonas aeruginosa |
E224D | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
H278A | inactive mutant enzyme | Pseudomonas aeruginosa |
H278A | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
H278N | inactive mutant enzyme | Pseudomonas aeruginosa |
H278N | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
H278V | inactive mutant enzyme | Pseudomonas aeruginosa |
H278V | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
N160A | kcat/Km is 5625fold higher than the wild-type value | Pseudomonas aeruginosa |
N160A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
R185A | kcat/Km is 7627fold higher than the wild-type value | Pseudomonas aeruginosa |
R185A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
R185K | site-directed mutagenesis, unstable mutant enzyme that precipitates | Pseudomonas aeruginosa |
R185L | kcat/Km is 25000fold higher than the wild-type value | Pseudomonas aeruginosa |
R185L | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
R243A | kcat/Km is 150000fold higher than the wild-type value | Pseudomonas aeruginosa |
R243A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
R243K | kcat/Km is 150000fold higher than the wild-type value | Pseudomonas aeruginosa |
R243K | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
R243L | kcat/Km is 225000fold higher than the wild-type value | Pseudomonas aeruginosa |
R243L | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
R401A | kcat/Km is 56250fold higher than the wild-type value | Pseudomonas aeruginosa |
R401A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
R401L | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Pseudomonas aeruginosa | |
0.14 | - |
L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
0.14 | - |
L-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
1.1 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme E224A | Pseudomonas aeruginosa | |
1.1 | - |
L-arginine | pH 5.6, 25°C, mutant E224A | Pseudomonas aeruginosa | |
2.6 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243A | Pseudomonas aeruginosa | |
2.6 | - |
L-arginine | pH 5.6, 25°C, mutant R243A | Pseudomonas aeruginosa | |
2.7 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme N160A | Pseudomonas aeruginosa | |
2.7 | - |
L-arginine | pH 5.6, 25°C, mutant N160A | Pseudomonas aeruginosa | |
4.2 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme E224D | Pseudomonas aeruginosa | |
4.2 | - |
L-arginine | pH 5.6, 25°C, mutant E224D | Pseudomonas aeruginosa | |
4.8 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R185L | Pseudomonas aeruginosa | |
4.8 | - |
L-arginine | pH 5.6, 25°C, mutant R185L | Pseudomonas aeruginosa | |
6.9 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243K | Pseudomonas aeruginosa | |
6.9 | - |
L-arginine | pH 5.6, 25°C, mutant R243K | Pseudomonas aeruginosa | |
8.7 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R401A | Pseudomonas aeruginosa | |
8.7 | - |
L-arginine | pH 5.6, 25°C, mutant R401A | Pseudomonas aeruginosa | |
15 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R185A | Pseudomonas aeruginosa | |
15 | - |
L-arginine | pH 5.6, 25°C, mutant R185A | Pseudomonas aeruginosa | |
21 | - |
Nomega-methyl-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
21 | - |
Nomega-methyl-L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
38 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243L | Pseudomonas aeruginosa | |
38 | - |
L-arginine | pH 5.6, 25°C, mutant R243L | Pseudomonas aeruginosa | |
50 | - |
Nomega-amino-L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
50 | - |
Nomega-amino-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | increases the activity 6fold at 60 mM | Pseudomonas aeruginosa | |
MgCl2 | increases the activity 8fold at 20 mM | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | Pseudomonas aeruginosa | ADI catalyzes the first step of the energy-producing arginine degradation pathway found in certain microorganisms, which ultimately leads to ornithine, carbamate, and ATP, overview | L-citrulline + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa | P13981 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzyme | Pseudomonas aeruginosa |
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-arginine + H2O = L-citrulline + NH3 | residues Cys406, His278, and Asp166 and the contribution from the neighboring Asp280 are important in catalysis of the formation and hydrolysis of the Cys406-alkyluronium intermediate, structure and kinetic analysis | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + H2O | - |
Pseudomonas aeruginosa | L-citrulline + NH3 | - |
? | |
L-arginine + H2O | specific for | Pseudomonas aeruginosa | L-citrulline + NH3 | - |
? | |
L-arginine + H2O | ADI catalyzes the first step of the energy-producing arginine degradation pathway found in certain microorganisms, which ultimately leads to ornithine, carbamate, and ATP, overview | Pseudomonas aeruginosa | L-citrulline + NH3 | - |
? | |
additional information | no activity with agmatine | Pseudomonas aeruginosa | ? | - |
? | |
Nomega-amino-Arg + H2O | - |
Pseudomonas aeruginosa | L-citrulline + hydrazine | - |
? | |
Nomega-amino-L-arginine + H2O | - |
Pseudomonas aeruginosa | L-citrulline + hydrazine | - |
? | |
Nomega-methyl-Arg + H2O | - |
Pseudomonas aeruginosa | L-citrulline + methylamine | - |
? | |
Nomega-methyl-L-arginine + H2O | - |
Pseudomonas aeruginosa | L-citrulline + methylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | ADI C406A-L-arginine structure, overview | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
ADI | - |
Pseudomonas aeruginosa |
More | ADI belongs to the guanidino group-modifying enzyme superfamily, GMSF, which conserves backbone fold and a Cys-, His-, and Asp-based catalytic core | Pseudomonas aeruginosa |
PaADI | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity at 45°C | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00036 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R401L | Pseudomonas aeruginosa | |
0.00089 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243A | Pseudomonas aeruginosa | |
0.002 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243K | Pseudomonas aeruginosa | |
0.0021 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme E224A | Pseudomonas aeruginosa | |
0.0037 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme E224D | Pseudomonas aeruginosa | |
0.0072 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R401A | Pseudomonas aeruginosa | |
0.0084 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R185L | Pseudomonas aeruginosa | |
0.009 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243L | Pseudomonas aeruginosa | |
0.01 | - |
Nomega-methyl-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
0.021 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme N160A | Pseudomonas aeruginosa | |
0.089 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R185A | Pseudomonas aeruginosa | |
0.2 | - |
L-arginine | pH 5.6, 25°C, mutant R243L | Pseudomonas aeruginosa | |
0.3 | - |
L-arginine | pH 5.6, 25°C, mutant R243A | Pseudomonas aeruginosa | |
0.3 | - |
L-arginine | pH 5.6, 25°C, mutant R243K | Pseudomonas aeruginosa | |
0.8 | - |
L-arginine | pH 5.6, 25°C, mutant R401A | Pseudomonas aeruginosa | |
0.9 | - |
L-arginine | pH 5.6, 25°C, mutant E224D | Pseudomonas aeruginosa | |
1 | - |
Nomega-methyl-L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
1.8 | - |
L-arginine | pH 5.6, 25°C, mutant R185L | Pseudomonas aeruginosa | |
1.9 | - |
L-arginine | pH 5.6, 25°C, mutant E224A | Pseudomonas aeruginosa | |
5.9 | - |
L-arginine | pH 5.6, 25°C, mutant R185A | Pseudomonas aeruginosa | |
6.3 | - |
L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
6.3 | - |
L-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
7 | - |
Nomega-amino-L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
7 | - |
Nomega-amino-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
8 | - |
L-arginine | pH 5.6, 25°C, mutant N160A | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
assay at | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.6 | pH profiles of wild-type and mutant enzymes | Pseudomonas aeruginosa |