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Literature summary for 3.5.3.3 extracted from
- Cloning, expression and purification of pseudomonas putida ATCC12633 creatinase (2017), Avicenna J. Med. Biotechnol., 9, 169-175 .
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | recombinant production of creatinase for using in clinical measurement of serum or urine creatinine | Pseudomonas putida |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida ATCC 12633 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.54 | - |
pH and temperature not specified in the publication | Pseudomonas putida |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Pseudomonas putida |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme degrades creatinine in bacteria and provides a source of carbon and nitrogen | Pseudomonas putida |
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