Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834 and BL21 (DE3) cells | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
seleno-L-methionine-labelled enzyme, hanging drop vapor diffusion method, using either 0.1 M phosphate citrate, pH 4.2, 10% (w/v) PEG 3000, 0.2 M NaCl or 0.1 M HEPES, pH 7.5, 7% (w/v) PEG 8000, 8% (v/v) ethylene glycol | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
E235A | inactive | Arabidopsis thaliana |
E235Q | inactive | Arabidopsis thaliana |
H237A | inactive | Arabidopsis thaliana |
H241A | inactive | Arabidopsis thaliana |
K291A | inactive | Arabidopsis thaliana |
K291R | the mutant shows reduced activity compared to the wild type | Arabidopsis thaliana |
Q275A | inactive | Arabidopsis thaliana |
Y252F | the mutant shows reduced activity compared to the wild type | Arabidopsis thaliana |
Y287A | inactive | Arabidopsis thaliana |
Y287F | inactive | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.77 | - |
(S)-2-ureidoglycine | wild type enzyme, at pH 8.5 and 30°C | Arabidopsis thaliana | |
2.3 | - |
(S)-2-ureidoglycine | mutant enzyme Y252F, at pH 8.5 and 30°C | Arabidopsis thaliana | |
3.46 | - |
(S)-2-ureidoglycine | mutant enzyme K291R, at pH 8.5 and 30°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | contains Mn2+, the Mn2+ ion acts as a molecular anchor to bind (S)-ureidoglycine, and its binding mode dictates the enantioselectivity of the reaction | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30200 | - |
8 * 30200, SDS-PAGE | Arabidopsis thaliana |
200000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-ureidoglycine + H2O | Arabidopsis thaliana | - |
(S)-ureidoglycolate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8GXV5 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity column chromatography and Superdex 200 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-ureidoglycine + H2O | - |
Arabidopsis thaliana | (S)-ureidoglycolate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 30200, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
UGLYAH | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73 | - |
(S)-2-ureidoglycine | mutant enzyme Y252F, at pH 8.5 and 30°C | Arabidopsis thaliana | |
493 | - |
(S)-2-ureidoglycine | mutant enzyme K291R, at pH 8.5 and 30°C | Arabidopsis thaliana | |
761 | - |
(S)-2-ureidoglycine | wild type enzyme, at pH 8.5 and 30°C | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
(S)-2-ureidoglycine | mutant enzyme Y252F, at pH 8.5 and 30°C | Arabidopsis thaliana | |
143 | - |
(S)-2-ureidoglycine | mutant enzyme K291R, at pH 8.5 and 30°C | Arabidopsis thaliana | |
429 | - |
(S)-2-ureidoglycine | wild type enzyme, at pH 8.5 and 30°C | Arabidopsis thaliana |