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Literature summary for 3.5.3.22 extracted from

  • Elkins, J.M.; Clifton, I.J.; Hernandez, H.; Doan, L.X.; Robinson, C.V.; Schofield, C.J.; Hewitson, K.S.
    Oligomeric structure of proclavaminic acid amidino hydrolase: evolution of a hydrolytic enzyme in clavulanic acid biosynthesis (2002), Biochem. J., 366, 423-434.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.22

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method, crystal structures of the enzyme at 1.75 A and 2.45 A resolution. Crystals belong to space group P2(1), with unit cell dimensions a = 95.4 A, b = 81.9 A, c = 118.6 A beta = 95.5°, but following transfer into the sucrose solution they belong to C2, with unit cell dimensions a = 140.0 A, b = 79.0 A, c = 93.5 A and beta = 124.0° Streptomyces clavuligerus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
 (3R)-hydroxy-N-acetyl-L-arginine pH 9.0, 37°C Streptomyces clavuligerus
30
-
 N-acetyl-L-arginine pH 9.0, 37°C Streptomyces clavuligerus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ preferred metal activator Streptomyces clavuligerus
Mn2+ 50% of the activity with Co2+ as activator Streptomyces clavuligerus
additional information no activation by Ca2+, Fe2+ and Zn2+ Streptomyces clavuligerus
Ni2+ 50% of the activity with Co2+ as activator Streptomyces clavuligerus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33401
-
 6 * 33401 Streptomyces clavuligerus
199500
-
 gel filtration Streptomyces clavuligerus

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-hydroxy-N-acetyl-L-arginine + H2O
-
 Streptomyces clavuligerus ?
-
 ?
N-acetyl-L-arginine + H2O
-
 Streptomyces clavuligerus ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 33401 Streptomyces clavuligerus

Synonyms

Synonyms Comment Organism
proclavaminic acid amidino hydrolaseproclavaminic acid amidino hydrolase
-
 Streptomyces clavuligerus