Application | Comment | Organism |
---|---|---|
medicine | endogenous nitric oxide synthase inhibitor asymmetrical dimethylarginine (hydrolyzed by DDAH) is elevated in many patients and may contribute to the initiation and progression of their disease | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-4-(NG-methyl-guanidino)butanoic acid | incubation of lysed red blood cells, dose dependent inhibition of NG,NG-dimethyl-L-arginine degradation with the highest dose (1 mM) reversing the pattern to a net increase in NG,NG-dimethyl-L-arginine concentration for the 1- and 3h time points | Rattus norvegicus | |
aprotinin inhibitor | NG,NG-dimethyl-L-arginine release from freeze-thaw whole blood 34% inhibited | Rattus norvegicus | |
leupeptin | NG,NG-dimethyl-L-arginine release from freeze-thaw whole blood 55% inhibited | Rattus norvegicus | |
additional information | incubation of lysed red blood cells with EDTA or glucose failed to attenuate the degradation of NG,NG-dimethyl-L-arginine, incubation of whole blood cells with arginine methyltransferase inhibitors does not reduce NG,NG-dimethyl-L-arginine release | Rattus norvegicus | |
PMSF | NG,NG-dimethyl-L-arginine release from freeze-thaw whole blood 57% inhibited | Rattus norvegicus | |
protease inhibitor | NG,NG-dimethyl-L-arginine release from freeze-thaw whole blood 25-85% inhibited | Rattus norvegicus | |
Zn2+ | incubation of lysed red blood cells, diminished hydrolyis of NG,NG-dimethyl-L-arginine by addition of physiologically relevant concentrations (0.02 mM) | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Sprague-Dawley rat | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NG,NG-dimethyl-L-arginine + H2O | - |
Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DDAH | - |
Rattus norvegicus |
dimethylarginine dimethylaminohydrolase | - |
Rattus norvegicus |