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Literature summary for 3.5.3.18 extracted from
- Inactivation of two diverse enzymes in the amidinotransferase superfamily by 2-chloroacetamidine: dimethylargininase and peptidylarginine deiminase (2005), Biochemistry, 44, 13744-13752.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | 2-chloroacetamidine may potentially find wide applicability as a general pharmacophore, useful in delineating characteristics of the amidinotransferase superfamily | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C249S | active-site Cys249 residue | Pseudomonas aeruginosa |
| H162G | active-site His162 residue | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-chloroacetamidine | irreversible inhibition in a time- and concentration-dependent manner | Pseudomonas aeruginosa |  |
| additional information | not inhibited by 2-chloroacetamide | Pseudomonas aeruginosa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30500 | - |
calculated from the protein sequence of wild-type DDAH, after removal of the N-terminal methionine residue | Pseudomonas aeruginosa |
| 30500 | - |
deconvoluted mass spectrum of a control reaction mixture that included wild-type DDAH but no inactivator | Pseudomonas aeruginosa |
| 30560 | - |
fully inactivated wild-type DDAH by 2-chloroacetamidine | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9I4E3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to more than 95% homogeneity | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DDAH | - |
Pseudomonas aeruginosa |
| dimethylarginine dimethylaminohydrolase | - |
Pseudomonas aeruginosa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
2-chloroacetamidine | - |
Pseudomonas aeruginosa | |
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Release 2023.1 (January 2023)
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