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Literature summary for 3.5.3.15 extracted from

  • Dreyton, C.J.; Knuckley, B.; Jones, J.E.; Lewallen, D.M.; Thompson, P.R.
    Mechanistic studies of protein arginine deiminase 2: evidence for a substrate-assisted mechanism (2014), Biochemistry, 53, 4426-4433.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 Nalpha-benzoyl-L-arginine methyl ester at pH 7.6 and 37°C Homo sapiens
0.27
-
 Nalpha-benzoyl-L-arginine ethyl ester at pH 7.6 and 37°C Homo sapiens
0.48
-
 Nalpha-benzoyl-L-arginine amide at pH 7.6 and 37°C Homo sapiens
1.6
-
 Nalpha-benzoyl-L-arginine at pH 7.6 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, the concentration of calcium that is required for half-maximal activity is 0.2 mM Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein L-arginine + H2O Homo sapiens
-
 protein L-citrulline + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y2J8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose resin, column chromatography, and gel filtration Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, in 20 mM Tris-HCl, pH 7.6, 1 mM EDTA, 500 mM NaCl, 0.5M tris(2-carboxyethyl) phosphine, and 10% (v/v) glycerol, several months, the enzyme remains stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information nearly no activity with free L-arginine, L-agmatine, histone H3, and histone H4 Homo sapiens ?
-
 ?
Nalpha-benzoyl-L-arginine + H2O
-
 Homo sapiens Nalpha-benzoyl-L-citrulline + NH3
-
 ?
Nalpha-benzoyl-L-arginine amide + H2O
-
 Homo sapiens Nalpha-benzoyl-L-citrulline amide + NH3
-
 ?
Nalpha-benzoyl-L-arginine ethyl ester + H2O 100% activity Homo sapiens Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?
Nalpha-benzoyl-L-arginine methyl ester + H2O
-
 Homo sapiens Nalpha-benzoyl-L-citrulline methyl ester + NH3
-
 ?
protein L-arginine + H2O
-
 Homo sapiens protein L-citrulline + NH3
-
 ?

Synonyms

Synonyms Comment Organism
PAD2
-
 Homo sapiens
protein arginine deiminase 2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
 Nalpha-benzoyl-L-arginine amide at pH 7.6 and 37°C Homo sapiens
0.43
-
 Nalpha-benzoyl-L-arginine methyl ester at pH 7.6 and 37°C Homo sapiens
0.63
-
 Nalpha-benzoyl-L-arginine at pH 7.6 and 37°C Homo sapiens
3.2
-
 Nalpha-benzoyl-L-arginine ethyl ester at pH 7.6 and 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.39
-
 Nalpha-benzoyl-L-arginine at pH 7.6 and 37°C Homo sapiens
0.68
-
 Nalpha-benzoyl-L-arginine amide at pH 7.6 and 37°C Homo sapiens
1.8
-
 Nalpha-benzoyl-L-arginine methyl ester at pH 7.6 and 37°C Homo sapiens
11.7
-
 Nalpha-benzoyl-L-arginine ethyl ester at pH 7.6 and 37°C Homo sapiens