Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M CaCl2, 20% (w/v) polyethylene glycol 3350 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
x * 76000, His-tagged enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein L-arginine + H2O | Homo sapiens | - |
protein L-citrulline + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9ULC6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-charged HiTrap column chromatography and Sephacryl S-200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein L-arginine + H2O | - |
Homo sapiens | protein L-citrulline + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, His-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PAD1 | - |
Homo sapiens |
peptidylarginine deiminase type I | - |
Homo sapiens |