Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.13 extracted from

  • Marti-Arbona, R.; Raushel, F.M.
    Mechanistic characterization of N-formimino-L-glutamate iminohydrolase from Pseudomonas aeruginosa (2006), Biochemistry, 45, 14256-14262.
    View publication on PubMed
Search for more literature for 3.5.3.13

Protein Variants

Protein Variants Comment Organism
E235A lower activity compared to the wild type enzyme Pseudomonas aeruginosa
E235Q lower activity compared to the wild type enzyme Pseudomonas aeruginosa
H269A about 5fold lower activity compared to the wild type enzyme Pseudomonas aeruginosa
H269C about 5fold lower activity compared to the wild type enzyme Pseudomonas aeruginosa
H269N about 5fold lower activity compared to the wild type enzyme Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
dipicolinate zinc can be removed by dialysis against the metal chelator dipicolinate with the complete loss of catalytic activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 N-formimino-L-glutamate wild type enzyme, at pH 8.0 and 30°C Pseudomonas aeruginosa
0.66
-
 N-formimino-L-glutamate mutant enzyme E235D, at pH 8.0 and 30°C Pseudomonas aeruginosa
1.2
-
 N-formimino-L-glutamate Cu2+-reconstituted wild type enzyme, at pH 8.0 and 30°C Pseudomonas aeruginosa
2.1
-
 N-formimino-L-glutamate Cd2+-reconstituted wild type enzyme, at pH 8.0 and 30°C Pseudomonas aeruginosa
2.2
-
 N-formimino-L-glutamate mutant enzyme E235A, at pH 8.0 and 30°C Pseudomonas aeruginosa
2.7
-
 N-formimino-L-glutamate Ni2+-reconstituted wild type enzyme, at pH 8.0 and 30°C Pseudomonas aeruginosa
19
-
 N-formimino-L-glutamate mutant enzyme E235Q, at pH 8.0 and 30°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ can replace Zn2+ Pseudomonas aeruginosa
Cu2+ can replace Zn2+ Pseudomonas aeruginosa
Ni2+ can replace Zn2+ Pseudomonas aeruginosa
Zn2+ contains 1.2 equivalents of Zn2+ per protein subunit, essential for activity Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 strain PA01
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, High Load 26/60 Superdex 200 gel filtration, and Resource Q anion exchange column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formimino-L-glutamate + H2O
-
 Pseudomonas aeruginosa N-formyl-L-glutamate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
HutF
-
 Pseudomonas aeruginosa
N-formimino-L-glutamate iminohydrolase
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 N-formimino-L-glutamate mutant enzyme E235D, at pH 8.0 and 30°C Pseudomonas aeruginosa
0.1
-
 N-formimino-L-glutamate mutant enzyme E235A, at pH 8.0 and 30°C Pseudomonas aeruginosa
0.12
-
 N-formimino-L-glutamate mutant enzyme E235Q, at pH 8.0 and 30°C Pseudomonas aeruginosa
8.5
-
 N-formimino-L-glutamate Cu2+-reconstituted wild type enzyme, at pH 8.0 and 30°C Pseudomonas aeruginosa
13.2
-
 N-formimino-L-glutamate Zn2+-reconstituted wild type enzyme, at pH 8.0 and 30°C Pseudomonas aeruginosa
21.3
-
 N-formimino-L-glutamate Ni2+-reconstituted wild type enzyme, at pH 8.0 and 30°C Pseudomonas aeruginosa
31
-
 N-formimino-L-glutamate Cd2+-reconstituted wild type enzyme, at pH 8.0 and 30°C Pseudomonas aeruginosa