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Literature summary for 3.5.3.13 extracted from
- N-Formimino-L-glutamate iminohydrolase from histidine-adapted Pseudomonas (1972), J. Biol. Chem., 247, 1605-1609.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Tetranitromethane | 98% inhibition | Pseudomonas aeruginosa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
formiminoglutamate | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, sedimentation equilibrium experiment at 20000 rpm | Pseudomonas aeruginosa |
| 48000 | - |
2 * 48000, sedimentation equilibrium experiment at 22000 rpm | Pseudomonas aeruginosa |
| 53000 | - |
2 * 53000, SDS-PAGE | Pseudomonas aeruginosa |
| 96000 | - |
gel filtration, sedimentation equilibrium experiment at 6000 rpm | Pseudomonas aeruginosa |
| 100000 | - |
gel filtration, sedimentation equilibrium experiment at 13000 rpm | Pseudomonas aeruginosa |
| 108000 | - |
gel filtration, sedimentation equilibrium experiment at 12000 rpm | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formimino-L-glutamate + H2O | Pseudomonas aeruginosa | - |
N-formyl-L-glutamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.7 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | formimino-L-aspartate and formiminoglycine are no substrates | Pseudomonas aeruginosa | ? | - |
? | |
| N-formimino-L-glutamate + H2O | - |
Pseudomonas aeruginosa | N-formyl-L-glutamate + NH3 | - |
? | |
| N-formimino-L-glutamic acid + H2O | - |
Pseudomonas aeruginosa | N-formyl-L-glutamic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 53000, SDS-PAGE | Pseudomonas aeruginosa |
| dimer | 2 * 45000, sedimentation equilibrium experiment at 20000 rpm | Pseudomonas aeruginosa |
| dimer | 2 * 48000, sedimentation equilibrium experiment at 22000 rpm | Pseudomonas aeruginosa |
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