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Literature summary for 3.5.3.13 extracted from

  • Hu, L.; Mulfinger, L.M.; Phillips, A.T.
    Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida (1987), J. Bacteriol., 169, 4696-4702.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.13

Cloned(Commentary)

Cloned (Comment) Organism
pMCl, entire group of hut genes from P. putida ATCC 12633 cloned into Escherichia coli , hutF encodes FIGLU iminohydrolase Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-formimino-L-glutamate + H2O Pseudomonas aeruginosa
-
 N-formyl-L-glutamate + NH3
-
 ?
N-formimino-L-glutamate + H2O Pseudomonas putida
-
 N-formyl-L-glutamate + NH3
-
 ?
N-formimino-L-glutamate + H2O Streptomyces coelicolor
-
 N-formyl-L-glutamate + NH3
-
 ?
N-formimino-L-glutamate + H2O Comamonas testosteroni
-
 N-formyl-L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas putida
-
 ATCC 12633
-
Streptomyces coelicolor
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
 wild type PRS1, inducer N-formimino-L-glutamate Pseudomonas putida
0.005
-
 wild type PRS1, inducer N-formyl-L-glutamate Pseudomonas putida
0.005
-
 wild type PRS1 Pseudomonas putida
0.015
-
 wild type PRS1, inducer urocanate Pseudomonas putida
0.06
-
 strain PS15(hutU) Pseudomonas putida
0.066
-
 wild type PRS1, inducer histidine Pseudomonas putida
0.086
-
 strain LH13(hutC) Pseudomonas putida
0.09
-
 strain LH12(hutC) Pseudomonas putida
0.093
-
 strain LH11(hutC) Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formimino-L-glutamate + H2O
-
 Pseudomonas aeruginosa N-formyl-L-glutamate + NH3
-
 ?
N-formimino-L-glutamate + H2O
-
 Pseudomonas putida N-formyl-L-glutamate + NH3
-
 ?
N-formimino-L-glutamate + H2O
-
 Streptomyces coelicolor N-formyl-L-glutamate + NH3
-
 ?
N-formimino-L-glutamate + H2O
-
 Comamonas testosteroni N-formyl-L-glutamate + NH3
-
 ?
N-formimino-L-glutamic acid + H2O
-
 Pseudomonas aeruginosa N-formyl-L-glutamic acid + NH3
-
 ?
N-formimino-L-glutamic acid + H2O
-
 Pseudomonas putida N-formyl-L-glutamic acid + NH3
-
 ?
N-formimino-L-glutamic acid + H2O
-
 Streptomyces coelicolor N-formyl-L-glutamic acid + NH3
-
 ?
N-formimino-L-glutamic acid + H2O
-
 Comamonas testosteroni N-formyl-L-glutamic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
FIGLU-iminohydrolase
-
 Pseudomonas putida
FIGLUase
-
 Pseudomonas putida