Any feedback?
Literature summary for 3.5.3.12 extracted from
- Listeria monocytogenes aguA1, but not aguA2, encodes a functional agmatine deiminase: biochemical characterization of its catalytic properties and roles in acid tolerance (2013), J. Biol. Chem., 288, 26606-26615.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells | Listeria monocytogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C157G | the mutant of inactive isoform AguA2 shows wild type enzyme activity | Listeria monocytogenes |
| C356A | inactive | Listeria monocytogenes |
| D218A | inactive | Listeria monocytogenes |
| D218E | inactive | Listeria monocytogenes |
| D94A | inactive | Listeria monocytogenes |
| D94E | inactive | Listeria monocytogenes |
| E155A | inactive | Listeria monocytogenes |
| E155D | inactive | Listeria monocytogenes |
| G157C | inactive | Listeria monocytogenes |
| H216A | inactive | Listeria monocytogenes |
| H216R | inactive | Listeria monocytogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Listeria monocytogenes |  |
| Cu2+ | - |
Listeria monocytogenes | |
| additional information | no inhibitory effect on isoform AguA1 activity with Fe3+, Mg2+, and Ni2+ | Listeria monocytogenes | |
| Zn2+ | - |
Listeria monocytogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
agmatine | at 25°C and pH 7.5 | Listeria monocytogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46800 | - |
x * 46800, calculated from amino acid sequence | Listeria monocytogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | Listeria monocytogenes | - |
N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | Listeria monocytogenes 10403S | - |
N-carbamoylputrescine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Listeria monocytogenes | - |
- |
- |
| Listeria monocytogenes 10403S | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-chelated agarose gel column chromatography | Listeria monocytogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, purified enzyme, 6 h, 80% loss of activity | Listeria monocytogenes |
| 4°C, purified enzyme, at least 1 month, 15% loss of activity | Listeria monocytogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | - |
Listeria monocytogenes | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | - |
Listeria monocytogenes 10403S | N-carbamoylputrescine + NH3 | - |
? | |
| additional information | L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity | Listeria monocytogenes | ? | - |
? | |
| additional information | L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity | Listeria monocytogenes 10403S | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46800, calculated from amino acid sequence | Listeria monocytogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgDI | - |
Listeria monocytogenes |
| AguA1 | - |
Listeria monocytogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Listeria monocytogenes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 35 | about 65% activity at 5°C, about 90% activity at 15°C, 100% activity at 25°C, less than 40% activity at 35°C | Listeria monocytogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34.28 | - |
agmatine | at 25°C and pH 7.5 | Listeria monocytogenes | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Listeria monocytogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 10.5 | more than 85% activity between pH 3.5 and 10.5 | Listeria monocytogenes |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
at 25°C and pH 7.5 | Listeria monocytogenes | Cu2+ | |
| 0.25 | - |
at 25°C and pH 7.5 | Listeria monocytogenes | Zn2+ | |
| 2.89 | - |
at 25°C and pH 7.5 | Listeria monocytogenes | Co2+ | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Listeria monocytogenes | transcription is significantly induced at pH 5.0 | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | deletion of aguA1 significantly impairs Listeria monocytogenes survival both in gastric fluid at pH 2.5 and in mouse stomach | Listeria monocytogenes |
| physiological function | the enzyme mediates acid tolerance in Listeria monocytogenes | Listeria monocytogenes |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 53 | - |
agmatine | at 25°C and pH 7.5 | Listeria monocytogenes | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
