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Literature summary for 3.5.3.12 extracted from
- Purification of monomeric agmatine iminohydrolase from soybean (1991), Biochem. Biophys. Res. Commun., 174, 32-36.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Glycine max |  |
| Co2+ | - |
Zea mays | |
| Cu2+ | - |
Glycine max | |
| Cu2+ | - |
Zea mays | |
| iodoacetamide | - |
Glycine max | |
| iodoacetamide | - |
Zea mays | |
| N-ethylmaleimide | - |
Glycine max | |
| N-ethylmaleimide | - |
Zea mays | |
| p-chloromercuribenzoate | - |
Glycine max | |
| p-chloromercuribenzoate | - |
Zea mays | |
| spermidine | - |
Oryza sativa | |
| spermine | - |
Oryza sativa | |
| Zn2+ | - |
Glycine max | |
| Zn2+ | - |
Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
agmatine | - |
Zea mays | |
| 2.5 | - |
agmatine | - |
Glycine max | |
| 15 | - |
agmatine | - |
Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Zea mays | 5829 | - |
| cytosol | - |
Glycine max | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Glycine max |
| 85000 | - |
- |
Zea mays |
| 183000 | - |
- |
Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | Zea mays | - |
N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | Glycine max | - |
N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | Oryza sativa | - |
N-carbamoylputrescine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
| Oryza sativa | - |
- |
- |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
- |
Glycine max |
- |
Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| axis | - |
Glycine max | - |
| seedling | - |
Zea mays | - |
| seedling | - |
Glycine max | - |
| seedling | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 172.2 | - |
- |
Glycine max |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme lost 30% of the activity in 3 days | Zea mays |
| 4°C, purified enzyme lost 30% of the activity in 3 days | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | - |
Zea mays | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | - |
Glycine max | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | - |
Oryza sativa | N-carbamoylputrescine + NH3 | - |
? | |
| additional information | arginine, creatine and citrulline are no substrates | Zea mays | ? | - |
? | |
| additional information | arginine, creatine and citrulline are no substrates | Glycine max | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Zea mays |
| dimer | - |
Oryza sativa |
| monomer | 1 * 70000, SDS-PAGE | Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Oryza sativa |
| 50 | - |
- |
Glycine max |
| 60 | - |
- |
Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Oryza sativa |
| 6.5 | - |
- |
Zea mays |
| 6.5 | - |
- |
Glycine max |
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