General Stability | Organism |
---|---|
highly unstable even in presence of glycerol, dithiothreitol and Mg2+, freeze-thawing and dialysis lead to considerable loss of activity | Lathyrus sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration, PAGE | Lathyrus sativus |
56000 | - |
gel filtration, Sephadex G-200 | Lathyrus sativus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | Lathyrus sativus | - |
N-carbamoylputrescine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lathyrus sativus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lathyrus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Lathyrus sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
- |
Lathyrus sativus |
Storage Stability | Organism |
---|---|
4°C purified enzyme lost all component activities within 48 h | Lathyrus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | - |
Lathyrus sativus | N-carbamoylputrescine + NH3 | - |
? | |
agmatine + H2O | multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase | Lathyrus sativus | N-carbamoylputrescine + NH3 | - |
? | |
agmatine + ornithine + H2O + phosphate | - |
Lathyrus sativus | putrescine + citrulline + NH3 + phosphate | - |
? | |
carbamoyl phosphate + ADP + H2O | - |
Lathyrus sativus | ATP + CO2 + NH3 | - |
r | |
carbamoyl phosphate + ornithine | - |
Lathyrus sativus | citrulline + phosphate | - |
r | |
N-carbamoylputrescine + phosphate | - |
Lathyrus sativus | putrescine + carbamoyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, single polypeptide chain, SDS-PAGE | Lathyrus sativus |
Synonyms | Comment | Organism |
---|---|---|
putrescine synthase | - |
Lathyrus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
putrescine transcarbamylase and carbamate kinase function | Lathyrus sativus |
8 | - |
ornithine transcarbamylase function | Lathyrus sativus |
8.8 | - |
agmatine iminohydrolase function | Lathyrus sativus |