Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.11 extracted from

  • Miller, D.; Xu, H.; White, R.H.
    A new subfamily of agmatinases present in methanogenic Archaea is Fe(II) dependent (2012), Biochemistry, 51, 3067-3078.
    View publication on PubMed
Search for more literature for 3.5.3.11

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol the enzyme requires the presence of dithiothreitol for maximum activity Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
C136A mutant has 90% of the activity compared to wild type in the presence of Fe(II). As the wild-type enzyme the mutant enzyme requires dithiothreitol for activity Methanocaldococcus jannaschii
C151S mutant has 6% of the activity compared to wild type in the presence of Fe(II). When dithiothreitol is present in the reaction the mutant shows 24% of the wild type activity Methanocaldococcus jannaschii
C229A mutant has 92% of the activity compared to wild type in the presence of Fe(II) Methanocaldococcus jannaschii
C71S mutant has 4% of the activity compared to wild type in the presence of Fe(II). When dithiothreitol is present in the reaction the mutant shows 24% of the wild type activity Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid the enzyme is rendered inactive when the purified enzyme is incubated with dithiothreitol followed by excess iodoacetic acid Methanocaldococcus jannaschii
L-arginine 44% inhibition at at 16.7 mM Methanocaldococcus jannaschii
N-ethylmaleinimide the enzyme is rendered inactive when the purified enzyme is incubated with dithiothreitol followed by excess N-ethylmaleimide Methanocaldococcus jannaschii
putrescine 43% inhibition at 8.3 mM, 60% inhibition at 16.7 mM Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 agmatine pH 7.5, 70°C, wild-type enzyme Methanocaldococcus jannaschii
5.8
-
 agmatine pH 7.5, 70°C, mutant enzyme C229A Methanocaldococcus jannaschii
6.2
-
 agmatine pH 7.5, 70°C, mutant enzyme C151S Methanocaldococcus jannaschii
9.2
-
 agmatine pH 7.5, 70°C, mutant enzyme C136A Methanocaldococcus jannaschii
11
-
 agmatine pH 7.5, 70°C, mutant enzyme C71S Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe(II)-dependent agmatinase. Requires the presence of 4 equivalents of Fe(II) for maximum activity Methanocaldococcus jannaschii
Mn2+ supports activity at 15% of the activation by Fe2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Methanocaldococcus jannaschii
190000
-
 gel filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57757
-
-
Methanocaldococcus jannaschii DSM 2661 Q57757
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
 pH 7.5, 70°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O
-
 Methanocaldococcus jannaschii putrescine + urea
-
 ?
agmatine + H2O
-
 Methanocaldococcus jannaschii DSM 2661 putrescine + urea
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
MJ0309 locus name Methanocaldococcus jannaschii
SpeB
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0075
-
 agmatine pH 7.5, 70°C, mutant enzyme C71S Methanocaldococcus jannaschii
0.0095
-
 agmatine pH 7.5, 70°C, mutant enzyme C151S Methanocaldococcus jannaschii
0.023
-
 agmatine pH 7.5, 70°C, mutant enzyme C136A Methanocaldococcus jannaschii
0.032
-
 agmatine pH 7.5, 70°C, wild-type enzyme Methanocaldococcus jannaschii
0.041
-
 agmatine pH 7.5, 70°C, mutant enzyme C229A Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 no activity is detected at pH 6.5 and the measured activity steadily increases to pH 10.0. Higher pH values are tested but the observation of activity is dependent on the buffer used and are not reported here. Agmatine shows no hydrolysis in the pH 10 and 11 buffers and assay conditions used Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00069
-
 agmatine pH 7.5, 70°C, mutant enzyme C71S Methanocaldococcus jannaschii
0.0015
-
 agmatine pH 7.5, 70°C, mutant enzyme C151S Methanocaldococcus jannaschii
0.0025
-
 agmatine pH 7.5, 70°C, mutant enzyme C136A Methanocaldococcus jannaschii
0.0077
-
 agmatine pH 7.5, 70°C, mutant enzyme C229A Methanocaldococcus jannaschii
0.013
-
 agmatine pH 7.5, 70°C, wild-type enzyme Methanocaldococcus jannaschii