Literature summary for 3.5.3.11 extracted from

Alarcon, R.; Orellana, M.S.; Neira, B.; Uribe, E.; Garcia, J.R.; Carvajal, N.
Mutational analysis of substrate recognition by human arginase type I -agmatinase activity of the N130D variant (2006), FEBS J., 273, 5625-5631.

Search for more literature for 3.5.3.11

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain JM109	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
putrescine	linear competitive inhibitor	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.4	-	agmatine	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	totally dependent on Mn2+ for catalytic activity	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
120000	-	SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
agmatine + H2O	the N130D mutant of arginase type I is able to cleave not only arginine but also agmatine (1-amino-4-guanidinobutane)	Homo sapiens	putrescine + urea	-	?

Synonyms

Synonyms	Comment	Organism
N130D variant of arginase type I	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3	-	agmatine	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.3	-	putrescine	-	Homo sapiens

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Release 2023.1 (January 2023)