Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.1 extracted from

  • Glazer, E.S.; Stone, E.M.; Zhu, C.; Massey, K.L.; Hamir, A.N.; Curley, S.A.
    Bioengineered human arginase I with enhanced activity and stability controls hepatocellular and pancreatic carcinoma xenografts (2011), Transl. Oncol., 4, 138-146.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine Co2+ substitution of the Mn2+ metal cofactor confers more than 10fold higher catalytic activity and 5fold greater stability. Based on the hypothesis that the Co-ArgI enzyme would decrease tumor burden by systemic elimination of L-arg in a murine model, Co-hArgI was conjugated to 5-kDa PEG to enhance circulation persistence and applied as monotherapy for hepatocellular carcinoma and pancreatic carcinoma in vitro and in vivo murine xenografts. Weekly treatment of 8 mg/kg Co-hArgI-PEG effectively controls human HepG2 and Panc-1 tumor xenografts. Both cell lines underwent apoptosis in vitro with significant increased expression of activated caspase-3 and showed evidence of autophagy in vitro and in vivo Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P05089
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Synonyms

Synonyms Comment Organism
ArgI
-
Homo sapiens