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Literature summary for 3.5.3.1 extracted from

  • Srivastava, A.; Dwivedi, N.; Samanta, U.; Sau, A.K.
    Insight into the role of a unique SSEHA motif in the activity and stability of Helicobacter pylori arginase (2011), IUBMB Life, 63, 1027-1036.
    View publication on PubMed
Search for more literature for 3.5.3.1

Protein Variants

Protein Variants Comment Organism
A92S mutation of A92 to its analogous residues in other arginases individually enhances the catalytic activity Helicobacter pylori
D116A no detectable activity Helicobacter pylori
E90A residue E90 is important for catalytic activity and plays a crucial role in retaining the metal ion at the active site Helicobacter pylori
E90A/H118A no detectable activity Helicobacter pylori
H91A residue E90 is important for catalytic activity and plays a crucial role in retaining the metal ion at the active site Helicobacter pylori
S88G mutation of Ser88 to its analogous residues in other arginases individually enhances the catalytic activity Helicobacter pylori
S88G/A92S mutation of S88 and A92 to its analogous residues in other arginases individually enhances the catalytic activity Helicobacter pylori
S89G 1.5fold decrease in catalytic activity Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
 L-arginine mutant S89G, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 2.1 Helicobacter pylori
4.4
-
 L-arginine mutant H91A, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.3 Helicobacter pylori
5.2
-
 L-arginine mutant A92S, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 1.6 Helicobacter pylori
5.9
-
 L-arginine mutant A92S, Co2+-activated, pH 5.0, 37°C, Hill coefficient 1.9 Helicobacter pylori
5.9
-
 L-arginine mutant S89G, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.2 Helicobacter pylori
6.3
-
 L-arginine mutant S88G, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.0 Helicobacter pylori
6.4
-
 L-arginine mutant E90A, Co2+-activated, pH 5.0, 37°C, Hill coefficient 1.7 Helicobacter pylori
6.7
-
 L-arginine mutant S88G/A92S, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.0 Helicobacter pylori
6.7
-
 L-arginine wild-type, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 1.6 Helicobacter pylori
8.4
-
 L-arginine wild-type, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.1 Helicobacter pylori
8.7
-
 L-arginine mutant S88G, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 2.4 Helicobacter pylori
9.2
-
 L-arginine mutant S88G/A92S, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 2.2 Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Helicobacter pylori L-ornithine + urea
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
69
-
 melting temperature, apo-form of wild-type Helicobacter pylori
71
-
 melting temperature, holo-form of wild-type Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 L-arginine mutant E90A, Co2+-activated, pH 5.0, 37°C Helicobacter pylori
0.013
-
 L-arginine mutant H91A, Co2+-activated, pH 5.0, 37°C Helicobacter pylori
0.04
-
 L-arginine mutant S89G, Mn2+-activated, pH 5.0, 37°C Helicobacter pylori
0.067
-
 L-arginine wild-type, Mn2+-activated, pH 5.0, 37°C Helicobacter pylori
0.12
-
 L-arginine mutant S89G, Co2+-activated, pH 5.0, 37°C Helicobacter pylori
0.27
-
 L-arginine mutant S88G, Mn2+-activated, pH 5.0, 37°C Helicobacter pylori
0.28
-
 L-arginine mutant S88G/A92S, Mn2+-activated, pH 5.0, 37°C Helicobacter pylori
0.28
-
 L-arginine wild-type, Co2+-activated, pH 5.0, 37°C Helicobacter pylori
0.84
-
 L-arginine mutant A92S, Mn2+-activated, pH 5.0, 37°C Helicobacter pylori
0.92
-
 L-arginine mutant A92S, Co2+-activated, pH 5.0, 37°C Helicobacter pylori
1.01
-
 L-arginine mutant S88G, Co2+-activated, pH 5.0, 37°C Helicobacter pylori
1.37
-
 L-arginine mutant S88G/A92S, Co2+-activated, pH 5.0, 37°C Helicobacter pylori