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Literature summary for 3.5.3.1 extracted from

  • Lopez, V.; Alarcon, R.; Orellana, M.S.; Enriquez, P.; Uribe, E.; Martinez, J.; Carvajal, N.
    Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies. Alteration of substrate specificity by replacement of Asn149 with Asp (2005), FEBS J., 272, 4540-4548.
    View publication on PubMed
Search for more literature for 3.5.3.1

Protein Variants

Protein Variants Comment Organism
H120N 53% of wild-type activity, no alteration in KM-value Homo sapiens
H145N 95% of wild-type activity, no alteration in KM-value Homo sapiens
N149D almost complete loss of activity with L-arginine, gain of activity with agmatine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 L-arginine 37°C, pH 9.0, wild-type Homo sapiens
1.5
-
 L-arginine 37°C, pH 9.0, mutant H145N Homo sapiens
1.6
-
 L-arginine 37°C, pH 9.0, mutant H120N Homo sapiens
2.5
-
 agmatine 37°C, pH 9.0, mutant N149D Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation. after dialysis with EDTA, wild-type and mutant H145N contain 1.1 and 1.3 Mn2+ per subunit, resp., and are hlaf-.active. Mutant H120N contains less than 0.1 Mn2+ per subunit and is inactive Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isoform arginase II
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O for wild-type, no substrate. Hydrolysis by mutant N149D Homo sapiens 1,4-diaminobutane + urea
-
 ?
L-arginine + H2O
-
 Homo sapiens L-ornithine + urea
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
131
-
 L-arginine 37°C, pH 9.0, mutant H120N Homo sapiens
238
-
 L-arginine 37°C, pH 9.0, mutant H145N Homo sapiens
249
-
 L-arginine 37°C, pH 9.0, wild-type Homo sapiens