Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, 130% of initial activity | Microbacterium hydrocarbonoxydans | |
EDTA | 1 mM, 110% of initial activity | Microbacterium hydrocarbonoxydans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Microbacterium hydrocarbonoxydans |
Crystallization (Comment) | Organism |
---|---|
the catalytic triad of the (-)-gamma-lactamase is comprised of residues Ser98, Asp230, and His259 and an oxyanion hole is formed by Tyr32 and Met99 according to the alignment results | Microbacterium hydrocarbonoxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31300 | - |
2 * 31300, calculated, 2 * 33000, SDS-PAGE | Microbacterium hydrocarbonoxydans |
33000 | - |
2 * 31300, calculated, 2 * 33000, SDS-PAGE | Microbacterium hydrocarbonoxydans |
65000 | - |
PAGE | Microbacterium hydrocarbonoxydans |
65300 | - |
gel filtration | Microbacterium hydrocarbonoxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium hydrocarbonoxydans | E3SVS0 | additionally acts as amidase, EC 3.5.1.4 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,4S)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O | - |
Microbacterium hydrocarbonoxydans | (1S,4R)-4-aminocyclopent-2-ene-1-carbaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31300, calculated, 2 * 33000, SDS-PAGE | Microbacterium hydrocarbonoxydans |
Synonyms | Comment | Organism |
---|---|---|
(-)-gamma-lactamase | - |
Microbacterium hydrocarbonoxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Microbacterium hydrocarbonoxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
half-life 30 min | Microbacterium hydrocarbonoxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Microbacterium hydrocarbonoxydans |
8 | - |
- |
Microbacterium hydrocarbonoxydans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Microbacterium hydrocarbonoxydans | calculated | - |
4.7 |