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Literature summary for 3.5.2.B2 extracted from

  • Gao, S.; Huang, R.; Zhu, S.; Li, H.; Zheng, G.
    Identification and characterization of a novel (+)-gamma-lactamase from Microbacterium hydrocarbonoxydans (2016), Appl. Microbiol. Biotechnol., 100, 9543-9553 .
    View publication on PubMed
Search for more literature for 3.5.2.B2

Application

Application Comment Organism
drug development the enzyme can be a promising candidate of biocatalyst for industrial applications of highly valuable chiral pharmaceutical chemicals Microbacterium hydrocarbonoxydans
industry the enzyme can be a promising candidate of biocatalyst for industrial applications of highly valuable chiral pharmaceutical chemicals Microbacterium hydrocarbonoxydans

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli coli Rosetta (DE3) Microbacterium hydrocarbonoxydans

Protein Variants

Protein Variants Comment Organism
C111A (+)-gamma-lactamase activities is totally lost Microbacterium hydrocarbonoxydans
D13A mutant enzyme retains 10% (+)-gamma-lactamase activity Microbacterium hydrocarbonoxydans
K78A (+)-gamma-lactamase activities is totally lost Microbacterium hydrocarbonoxydans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.5 M, 5.5fold increase in activity Microbacterium hydrocarbonoxydans
Ni2+ 0.5 M, 5.5fold increase in activity Microbacterium hydrocarbonoxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 SDS-PAGE Microbacterium hydrocarbonoxydans

Organism

Organism UniProt Comment Textmining
Microbacterium hydrocarbonoxydans A0A0K0XHU0 similar in structure with bacterial isochorismatases
-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbacterium hydrocarbonoxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-azabicyclo[2.2.1]hept-5-en-3-one + H2O
-
 Microbacterium hydrocarbonoxydans (-)-2-azabicyclo[2.2.1]hept-5-en-3-one + (+)-4-amino-cyclopent-2-enecarboxylic acid
-
 ?
2-azabicyclo[2.2.1]hept-5-en-3-one + H2O 49.8% conversion, 99.7% enantiomeric excess, stereochemical preference to the (1R,4S)-enantiomer Microbacterium hydrocarbonoxydans (1R,4S)-4-aminocyclopent-2-ene-1-carboxylic acid + (1R,4S)-2-azabicyclo[2.2.1]hept-5-en-3-one
-
 ir
2-azabicyclo[2.2.1]heptan-3-one + H2O 100% conversion Microbacterium hydrocarbonoxydans (1S,3R)-3-aminocyclopentane-1-carboxylic acid + (1S,4R)-2-azabicyclo[2.2.1]heptan-3-one
-
 ir
2-methyl-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O 49.8% conversion, 99.8% enantiomeric excess Microbacterium hydrocarbonoxydans (1R,4S)-4-(methylamino)cyclopent-2-ene-1-carboxylic acid + (1R,4S)-2-methyl-2-azabicyclo[2.2.1]hept-5-en-3-one
-
 ir

Synonyms

Synonyms Comment Organism
Mh33H4-5540
-
 Microbacterium hydrocarbonoxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Microbacterium hydrocarbonoxydans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 35 20°C: about 95% of maximal activity, 35°C: about 50% of maximal activity, wild-type enzyme Microbacterium hydrocarbonoxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 5 h, about 55% loss of activity Microbacterium hydrocarbonoxydans
35
-
 5 h, about 85% loss of activity Microbacterium hydrocarbonoxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Microbacterium hydrocarbonoxydans
8
-
-
 Microbacterium hydrocarbonoxydans

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 80% of maximal activity, pH 10.0: about 80% of maximal activity, wild-type enzyme Microbacterium hydrocarbonoxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 30 min, the enzyme is relatively stable Microbacterium hydrocarbonoxydans