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Literature summary for 3.5.2.B2 extracted from

  • Kettle, A.J.; Carere, J.; Batley, J.; Benfield, A.H.; Manners, J.M.; Kazan, K.; Gardiner, D.M.
    A gamma-lactamase from cereal infecting Fusarium spp. catalyses the first step in the degradation of the benzoxazolinone class of phytoalexins (2015), Fungal Genet. Biol., 83, 1-9.
    View publication on PubMed
Search for more literature for 3.5.2.B2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Fusarium pseudograminearum
expression in Escherichia coli Fusarium graminearum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 1,3-benzoxazol-2(3H)-one pH 7.0, temperature not specified in the publication Fusarium pseudograminearum

Organism

Organism UniProt Comment Textmining
Fusarium graminearum I1R9E4
-
-
Fusarium graminearum ATCC MYA-4620 I1R9E4
-
-
Fusarium pseudograminearum K3VFR8
-
-
Fusarium pseudograminearum CS3096 K3VFR8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-benzoxazol-2(3H)-one + H2O complete conversion Fusarium pseudograminearum 2-aminophenol + CO2
-
 ?
1,3-benzoxazol-2(3H)-one + H2O complete conversion Fusarium graminearum 2-aminophenol + CO2
-
 ?
1,3-benzoxazol-2(3H)-one + H2O complete conversion Fusarium pseudograminearum CS3096 2-aminophenol + CO2
-
 ?
1,3-benzoxazol-2(3H)-one + H2O complete conversion Fusarium graminearum ATCC MYA-4620 2-aminophenol + CO2
-
 ?
6-methoxy-1,3-benzoxazol-2(3H)-one + H2O complete conversion Fusarium pseudograminearum 2-amino-5-methoxyphenol + CO2
-
 ?
6-methoxy-1,3-benzoxazol-2(3H)-one + H2O complete conversion Fusarium graminearum 2-amino-5-methoxyphenol + CO2
-
 ?
6-methoxy-1,3-benzoxazol-2(3H)-one + H2O complete conversion Fusarium pseudograminearum CS3096 2-amino-5-methoxyphenol + CO2
-
 ?
6-methoxy-1,3-benzoxazol-2(3H)-one + H2O complete conversion Fusarium graminearum ATCC MYA-4620 2-amino-5-methoxyphenol + CO2
-
 ?

Synonyms

Synonyms Comment Organism
FDB1
-
 Fusarium pseudograminearum
FDB1
-
 Fusarium graminearum
FGSG_00079
-
 Fusarium graminearum
FPSE_08124
-
 Fusarium pseudograminearum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
 1,3-benzoxazol-2(3H)-one pH 7.0, temperature not specified in the publication Fusarium pseudograminearum

Expression

Organism Comment Expression
Fusarium pseudograminearum increased expression upon treatment with benzoxazolin-2-one up
Fusarium graminearum increased expression upon treatment with benzoxazolin-2-one up

General Information

General Information Comment Organism
physiological function gene deletion mutant shows hyphal growth on solid media comparable to the wild-type and significantly reduced growth on media supplied with 1,3-benzoxazol-2(3H)-one and 6-methoxy-1,3-benzoxazol-2(3H)-one Fusarium pseudograminearum
physiological function gene deletion mutant shows hyphal growth on solid media comparable to the wild-type and significantly reduced growth on media supplied with 1,3-benzoxazol-2(3H)-one and 6-methoxy-1,3-benzoxazol-2(3H)-one Fusarium graminearum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22
-
 1,3-benzoxazol-2(3H)-one pH 7.0, temperature not specified in the publication Fusarium pseudograminearum