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Literature summary for 3.5.2.B2 extracted from

  • Xue, T.Y.; Xu, G.C.; Han, R.Z.; Ni, Y.
    Soluble expression of (+)-gamma-lactamase in Bacillus subtilis for the enantioselective preparation of abacavir precursor (2015), Appl. Biochem. Biotechnol., 176, 1687-1699.
    View publication on PubMed
Search for more literature for 3.5.2.B2

Application

Application Comment Organism
synthesis enantioselective resolution of 100 g/l 2-azabicyclo[2.2.1]hept-5-en-3-one, is achieved with 10 g/l dry cells, resulting in 55.2% conversion and 99% enantiomeric excess of the (-)-gamma-lactam, i.e. (1R,4S)-2-azabicyclo[2.2.1]hept-5-en-3-one Delftia acidovorans

Cloned(Commentary)

Cloned (Comment) Organism
-
 Delftia acidovorans

Protein Variants

Protein Variants Comment Organism
additional information compared with expression in Escherichia coli, recombinant lactamase shows improved protein solubility and catalytic activity when expressed in Bacillus subtilis 168 Delftia acidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.378
-
 (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one pH 9.0, 30°C Delftia acidovorans

Organism

Organism UniProt Comment Textmining
Delftia acidovorans A9BPK4
-
-
Delftia acidovorans DSM 14801 A9BPK4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O
-
 Delftia acidovorans (1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde
-
 ?
(1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O
-
 Delftia acidovorans DSM 14801 (1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde
-
 ?

Synonyms

Synonyms Comment Organism
Daci_0225
-
 Delftia acidovorans
Delm
-
 Delftia acidovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Delftia acidovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Delftia acidovorans