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Literature summary for 3.5.2.6 extracted from
- Molecular and biochemical characterization of the natural chromosome-encoded class A beta-lactamase from Pseudomonas luteola (2010), Antimicrob. Agents Chemother., 54, 45-51.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas luteola |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
piperacillin | pH and temperature not specified in the publication | Pseudomonas luteola |  |
| 0.008 | - |
penicillin G | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 0.014 | - |
ticarcillin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 0.02 | - |
amoxicillin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 0.021 | - |
cephalothin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 0.024 | - |
cefuroxime | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 0.035 | - |
ceftazidime | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 0.04 | - |
aztreonam | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 0.086 | - |
cefotaxime | pH and temperature not specified in the publication | Pseudomonas luteola | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28900 | - |
x * 28900, SDS-PAGE | Pseudomonas luteola |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas luteola | Q670S6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amoxicillin + H2O | - |
Pseudomonas luteola | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| aztreonam + H2O | - |
Pseudomonas luteola | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
| cefotaxime + H2O | - |
Pseudomonas luteola | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| ceftazidime + H2O | - |
Pseudomonas luteola | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| cefuroxime + H2O | - |
Pseudomonas luteola | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalothin + H2O | - |
Pseudomonas luteola | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| penicillin G + H2O | - |
Pseudomonas luteola | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| piperacillin + H2O | - |
Pseudomonas luteola | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ticarcillin + H2O | - |
Pseudomonas luteola | (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28900, SDS-PAGE | Pseudomonas luteola |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| blaLUT-1 | - |
Pseudomonas luteola |
| LUT-1 beta-lactamase | - |
Pseudomonas luteola |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
ceftazidime | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 1.5 | - |
aztreonam | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 5 | - |
ticarcillin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 8 | - |
piperacillin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 21 | - |
amoxicillin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 31 | - |
penicillin G | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 286 | - |
cefuroxime | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 452 | - |
cefotaxime | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 543 | - |
cephalothin | pH and temperature not specified in the publication | Pseudomonas luteola | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas luteola | calculated from sequence, isoelectric focusing | - |
6 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 40 | - |
ceftazidime | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 40 | - |
aztreonam | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 360 | - |
ticarcillin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 1050 | - |
amoxicillin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 1140 | - |
piperacillin | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 3870 | - |
penicillin G | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 5250 | - |
cefotaxime | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 11910 | - |
cefuroxime | pH and temperature not specified in the publication | Pseudomonas luteola | |
| 25860 | - |
cephalothin | pH and temperature not specified in the publication | Pseudomonas luteola | |
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