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Literature summary for 3.5.2.6 extracted from

  • de Castillo, M.C.; Islas, M.I.; de Nader, O.M.; de Ruiz-Holgado, A.P.
    Purification and characterization of beta-lactamase from Neisseria gonorrhoeae from clinical samples (2006), Rev. Latinoam. Microbiol., 43, 70-75.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 67% residual activity at 0.01 mM Neisseria gonorrhoeae
Cd2+ 24% residual activity at 0.01 mM Neisseria gonorrhoeae
Cu2+ 42% residual activity at 0.01 mM Neisseria gonorrhoeae
EDTA complete inhibition at 0.000001 mM Neisseria gonorrhoeae
Fe2+ 50% residual activity at 0.01 mM Neisseria gonorrhoeae
Fe3+ 24% residual activity at 0.01 mM Neisseria gonorrhoeae
Hg2+ complete inhibition at 0.000001 mM Neisseria gonorrhoeae
Mg2+ 97% residual activity at 0.01 mM Neisseria gonorrhoeae
Mn2+ 49% residual activity at 0.01 mM Neisseria gonorrhoeae
Ni2+ 28% residual activity at 0.01 mM Neisseria gonorrhoeae
p-chloromercuribenzoate 2% residual activity at 0.01 mM Neisseria gonorrhoeae
Zn2+ 6% residual activity at 0.01 mM Neisseria gonorrhoeae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
SDS-PAGE Neisseria gonorrhoeae

Organism

Organism UniProt Comment Textmining
Neisseria gonorrhoeae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G75 gel filtration, and DEAE-cellulose column chromatography Neisseria gonorrhoeae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.36
-
crude extract Neisseria gonorrhoeae
13.67
-
after 37.97fold purification Neisseria gonorrhoeae

Storage Stability

Storage Stability Organism
-30°C, 30 days, 79% loss of activity Neisseria gonorrhoeae
-70°C, 2 months, no loss of activity Neisseria gonorrhoeae
-70°C, 5 months, 22% loss of activity Neisseria gonorrhoeae
25°C, 25 days, complete loss of activity Neisseria gonorrhoeae
4°C, 5 days, 50% loss of activity Neisseria gonorrhoeae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephaloridine + H2O
-
Neisseria gonorrhoeae (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Neisseria gonorrhoeae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Neisseria gonorrhoeae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 55 residual activity of 82% after 25 min at 37°C and complete loss of activity after 1 min at 60°C or 5 min at 55°C Neisseria gonorrhoeae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.2 in 50 mM phosphate buffer Neisseria gonorrhoeae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2 8 at pH values higher than 7.2 a decrease of the enzyme activity is observed, having lost 50% at pH 8.0 Neisseria gonorrhoeae

pI Value

Organism Comment pI Value Maximum pI Value
Neisseria gonorrhoeae isoelectric focusing
-
5.4