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Literature summary for 3.5.2.6 extracted from

  • Ishii, Y.; Galleni, M.; Ma, L.; Frere, J.-M.; Yamaguchi, K.
    Biochemical characterisation of the CTX-M-14 beta-lactamase (2007), Int. J. Antimicrob. Agents, 29, 159-164.
    View publication on PubMed
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
aztreonam competitive inhibitor Escherichia coli
clavulanic acid
-
 Escherichia coli
additional information imipenem, meropenem or doripenem do not behave as inactivators Escherichia coli
tazobactam
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00011
-
 meropenem in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.00047
-
 Sulbactam in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.0008
-
 cefdinir in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.0019
-
 Imipenem in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.011
-
 benzylpenicillin in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.02
-
 piperacillin in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.025
-
 nitrocefin in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.025
-
 cefcapene in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.027
-
 cefalothin in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.041
-
 cefotaxime in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.041
-
 aztreonam in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.125
-
 cefaloridine in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.15
-
 doripenem in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
13
-
 ceftazidime in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27970
-
 mass spectrum analysis Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli TUM1121
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aztreonam + H2O poorly active against ceftazidime and aztreonam Escherichia coli [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
aztreonam + H2O poorly active against ceftazidime and aztreonam Escherichia coli TUM1121 [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
benzylpenicillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Escherichia coli TUM1121 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefaloridine + H2O
-
 Escherichia coli (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefalotin + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefcapene + H2O
-
 Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)pent-2-enoyl]amino}(carboxy)methyl]-5-[(carbamoyloxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefdinir + H2O
-
 Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(hydroxyimino)acetyl]amino}(carboxy)methyl]-5-ethenyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefepime + H2O
-
 Escherichia coli (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefepime + H2O
-
 Escherichia coli TUM1121 (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefotaxime + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefotaxime + H2O
-
 Escherichia coli TUM1121 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefpodoxime + H2O
-
 Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-5-(methoxymethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ceftazidime + H2O poorly active against ceftazidime and aztreonam Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
ceftazidime + H2O poorly active against ceftazidime and aztreonam Escherichia coli TUM1121 (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
ceftizoxime + H2O
-
 Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
doripenem + H2O
-
 Escherichia coli (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-({(3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
imipenem + H2O
-
 Escherichia coli (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Escherichia coli (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Escherichia coli (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
piperacillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
sulbactam + H2O
-
 Escherichia coli (2R,4S)-2-(carboxymethyl)-5,5-dimethyl-1,1-dioxo-1lambda~6~,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
CTX-M-14
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 Imipenem in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.007
-
 meropenem in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
0.04
-
 doripenem in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
20
-
 Sulbactam in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
39
-
 piperacillin in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
48
-
 benzylpenicillin in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
83
-
 cefdinir in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
110
-
 cefcapene in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
360
-
 nitrocefin in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
510
-
 cefalothin in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
1300
-
 cefaloridine in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli
1400
-
 cefotaxime in 50 mM phosphate buffer (pH 7.0), at 30°C Escherichia coli