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Literature summary for 3.5.2.6 extracted from

  • Doi, Y.; Poirel, L.; Paterson, D.L.; Nordmann, P.
    Characterization of a naturally occurring class D beta-lactamase from Achromobacter xylosoxidans (2008), Antimicrob. Agents Chemother., 52, 1952-1956.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information imipenem and cefoxitin do not induce OXA-114 expression Achromobacter xylosoxidans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Achromobacter xylosoxidans

Inhibitors

Inhibitors Comment Organism Structure
clavulanate
-
Achromobacter xylosoxidans
Sulbactam
-
Achromobacter xylosoxidans
tazobactam
-
Achromobacter xylosoxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
piperacillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
0.01
-
benzylpenicillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
0.015
-
cephalothin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
0.04
-
ticarcillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
2
-
Imipenem Km above 2 mM, in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
SDS-PAGE Achromobacter xylosoxidans

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans B3FIE3 strain CIP69598, previously Alcaligenes xylosoxidans
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and S-Sepharose column chromatography Achromobacter xylosoxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylpenicillin + H2O
-
Achromobacter xylosoxidans (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cephalothin + H2O
-
Achromobacter xylosoxidans (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
imipenem + H2O
-
Achromobacter xylosoxidans (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
additional information no hydrolysis of oxacillin, ceftazidime, cefotaxime, and cefoxitin Achromobacter xylosoxidans ?
-
?
piperacillin + H2O
-
Achromobacter xylosoxidans (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
ticarcillin + H2O
-
Achromobacter xylosoxidans (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Synonyms

Synonyms Comment Organism
OXA114 a class D beta-lactamase Achromobacter xylosoxidans
Oxacillinase
-
Achromobacter xylosoxidans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
benzylpenicillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
0.1
-
ticarcillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
0.5
-
cephalothin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans
0.5
-
piperacillin in 100 mM Tris-H2SO4, 300 mM K2SO4 (pH 7.0), at 30°C Achromobacter xylosoxidans

pI Value

Organism Comment pI Value Maximum pI Value
Achromobacter xylosoxidans isoelectric focusing
-
8.6