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Literature summary for 3.5.2.6 extracted from

  • Garau, G.; Bebrone, C.; Anne, C.; Galleni, M.; Frere, J.M.; Dideberg, O.
    A metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase CphA and its complex with biapenem (2005), J. Mol. Biol., 345, 785-795.
    View publication on PubMed
Search for more literature for 3.5.2.6

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of wild-type enzyme mutant enzyme N220G, and of the complex of wild-type enzyme with biapenem Aeromonas hydrophila

Protein Variants

Protein Variants Comment Organism
N220G kinetic parameters of the wild-type and N220G mutant enzymes are not significantly different Aeromonas hydrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 Imipenem 30°C, pH 6.5, mutant enzyme N220G Aeromonas hydrophila
0.118
-
 biapenem 30°C, pH 6.5, mutant enzyme N220G Aeromonas hydrophila
0.166
-
 biapenem 30°C, pH 6.5, wild-type enzyme Aeromonas hydrophila
0.34
-
 Imipenem 30°C, pH 6.5, wild-type enzyme Aeromonas hydrophila

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc enzyme contains one zinc ion Aeromonas hydrophila

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila P26918
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biapenem + H2O
-
 Aeromonas hydrophila (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate
-
 ?
imipenem + H2O
-
 Aeromonas hydrophila (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
carbapenemase CphA
-
 Aeromonas hydrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
160
-
 biapenem 30°C, pH 6.5, mutant enzyme N220G Aeromonas hydrophila
300
-
 biapenem 30°C, pH 6.5, wild-type enzyme Aeromonas hydrophila
390
-
 Imipenem 30°C, pH 6.5, mutant enzyme N220G Aeromonas hydrophila
1200
-
 Imipenem 30°C, pH 6.5, wild-type enzyme Aeromonas hydrophila