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Literature summary for 3.5.2.6 extracted from

  • Doi, Y.; Wachino, J.; Ishiguro, M.; Kurokawa, H.; Yamane, K.; Shibata, N.; Shibayama, K.; Yokoyama, K.; Kato, H.; Yagi, T.; Arakawa, Y.
    Inhibitor-sensitive AmpC beta-lactamase variant produced by an Escherichia coli clinical isolate resistant to oxyiminocephalosporins and cephamycins (2004), Antimicrob. Agents Chemother., 48, 2652-2658.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
clavulanic acid
-
 Escherichia coli
Sulbactam
-
 Escherichia coli
tazobactam
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 cefoxitin 37°C, pH 7.0 Escherichia coli
0.0057
-
 ceftazidime 37°C, pH 7.0 Escherichia coli
0.0062
-
 ampicillin 37°C, pH 7.0 Escherichia coli
0.021
-
 cefpirome 37°C, pH 7.0 Escherichia coli
0.031
-
 cefotaxime 37°C, pH 7.0 Escherichia coli
0.049
-
 cefepime 37°C, pH 7.0 Escherichia coli
0.1
-
 cephaloridine 37°C, pH 7.0 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q76DI4
-
-
Escherichia coli HKY28 Q76DI4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Escherichia coli HKY28 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefepime + H2O
-
 Escherichia coli (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefepime + H2O
-
 Escherichia coli HKY28 (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefotaxime + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefotaxime + H2O
-
 Escherichia coli HKY28 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Escherichia coli (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Escherichia coli HKY28 (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefpirome + H2O
-
 Escherichia coli (2S)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-5-[(6,7-dihydro-5H-cyclopenta[b]pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
ceftazidime + H2O
-
 Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephaloridine + H2O
-
 Escherichia coli (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephaloridine + H2O
-
 Escherichia coli HKY28 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?

Synonyms

Synonyms Comment Organism
AmpC beta-lactamase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.043
-
 cefoxitin 37°C, pH 7.0 Escherichia coli
0.084
-
 ceftazidime 37°C, pH 7.0 Escherichia coli
0.37
-
 cefotaxime 37°C, pH 7.0 Escherichia coli
0.43
-
 ampicillin 37°C, pH 7.0 Escherichia coli
1
-
 cefepime 37°C, pH 7.0 Escherichia coli
1.5
-
 cefpirome 37°C, pH 7.0 Escherichia coli
64
-
 cephaloridine 37°C, pH 7.0 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0087
-
 tazobactam 37°C, pH 7.0 Escherichia coli
0.0092
-
 Sulbactam 37°C, pH 7.0 Escherichia coli
0.32
-
 clavulanic acid 37°C, pH 7.0 Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli
-
-
 9.9