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Literature summary for 3.5.2.6 extracted from

  • Sun, T.; Nukaga, M.; Mayama, K.; Braswell, E.H.; Knox, J.R.
    Comparison of beta-lactamases of classes A and D: 1.5-A crystallographic structure of the class D OXA-1 oxacillinase (2003), Protein Sci., 12, 82-91.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
2.5-3 mg/ml purified recombinant enzyme in 10% PEG 8000, 0.05 M HEPES, pH 7.5, vapour diffusion sitting drop method, equilibration of 0.01 ml protein solution over a reservoir solution containing 20% PEG 8000, macro-seeding at 1.2-1.5 mg/ml, growth of prismatic crystals, X-ray diffraction structure determination and analysis at 1.5 A resolution, modeling Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28200
-
1 * 28200, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
class D enzyme OXA-1
-

Reaction

Reaction Comment Organism Reaction ID
a beta-lactam + H2O = a substituted beta-amino acid reaction mechanism, Lys70 is involved in catalysis, active site structure Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cloxacillin + H2O specific for Escherichia coli (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
oxacillin + H2O specific for Escherichia coli (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28200, SDS-PAGE Escherichia coli
More enzyme belongs to a subclass containing an enlarged OMEGA loop near the beta-lactam binding site, which projects into solvent and cannot interact directly with the substrate Escherichia coli

Synonyms

Synonyms Comment Organism
OXA-1
-
Escherichia coli
Oxacillinase
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli