Crystallization (Comment) | Organism |
---|---|
2.5-3 mg/ml purified recombinant enzyme in 10% PEG 8000, 0.05 M HEPES, pH 7.5, vapour diffusion sitting drop method, equilibration of 0.01 ml protein solution over a reservoir solution containing 20% PEG 8000, macro-seeding at 1.2-1.5 mg/ml, growth of prismatic crystals, X-ray diffraction structure determination and analysis at 1.5 A resolution, modeling | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28200 | - |
1 * 28200, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
class D enzyme OXA-1 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a beta-lactam + H2O = a substituted beta-amino acid | reaction mechanism, Lys70 is involved in catalysis, active site structure | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cloxacillin + H2O | specific for | Escherichia coli | (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
oxacillin + H2O | specific for | Escherichia coli | (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28200, SDS-PAGE | Escherichia coli |
More | enzyme belongs to a subclass containing an enlarged OMEGA loop near the beta-lactam binding site, which projects into solvent and cannot interact directly with the substrate | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
OXA-1 | - |
Escherichia coli |
Oxacillinase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |