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Literature summary for 3.5.2.6 extracted from

  • Hedberg, M.; Lindqvist, L.; Tuner, K.; Nord, C.E.
    Effect of clavulanic acid, sulbactam and tazobactam on three different beta-lactamases from Bacteroides uniformes, Clostridium butyricum and Fusobacterium nucleatum (1992), J. Antimicrob. Chemother., 30, 17-25.
    View publication on PubMed
Search for more literature for 3.5.2.6

Inhibitors

Inhibitors Comment Organism Structure
clavulanic acid inactivation in 30 min, IC50 0.00003 mM Bacteroides uniformis
clavulanic acid inactivation in 1-2 h Clostridium butyricum
clavulanic acid inactivation in 1-2 h Fusobacterium nucleatum
Sulbactam IC50 0.00018 mM Bacteroides uniformis
Sulbactam IC50 1-3 mM Clostridium butyricum
Sulbactam IC50 1-3 mM Fusobacterium nucleatum
tazobactam IC50 deoendent on enzyme concentration; inactivation after 1 h , IC50 0.00005 mM Bacteroides uniformis
tazobactam IC50 deoendent on enzyme concentration; inactivation after 1-2 h Clostridium butyricum
tazobactam IC50 deoendent on enzyme concentration; inactivation after 1-2 h Fusobacterium nucleatum

Organism

Organism UniProt Comment Textmining
Bacteroides uniformis
-
-
-
Clostridium butyricum
-
-
-
Fusobacterium nucleatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium butyricum
-
 Fusobacterium nucleatum
-
 Bacteroides uniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrocefin + H2O
-
 Clostridium butyricum (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Fusobacterium nucleatum (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Bacteroides uniformis (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00003
-
 inactivation in 30 min, IC50 0.00003 mM Bacteroides uniformis clavulanic acid
0.00005
-
 inactivation after 1 h , IC50 0.00005 mM Bacteroides uniformis tazobactam
0.00018
-
 IC50 0.00018 mM Bacteroides uniformis Sulbactam
1 3 IC50 1-3 mM Clostridium butyricum Sulbactam
1 3 IC50 1-3 mM Fusobacterium nucleatum Sulbactam