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Literature summary for 3.5.2.6 extracted from

  • Yang, A.; Bush, K.
    Biochemical characterization of the carbapenem-hydrolyzing beta-lactamse AsbM1 from Aeromonas sobria AER 14M; a member of a novel subgroup of metallo-beta-lactamases (1996), FEMS Microbiol. Lett., 137, 193-200.
    View publication on PubMed
Search for more literature for 3.5.2.6

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Aeromonas sobria
Cd2+
-
 Aeromonas sobria
EDTA
-
 Aeromonas sobria
p-chloromercuribenzoate
-
 Aeromonas sobria
Zn2+
-
 Aeromonas sobria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 carbenicillin
-
 Aeromonas sobria
0.065
-
 ampicillin
-
 Aeromonas sobria
0.16
-
 benzylpenicillin
-
 Aeromonas sobria
0.16
-
 biapenem
-
 Aeromonas sobria
0.23
-
 Imipenem
-
 Aeromonas sobria
0.63
-
 meropenem
-
 Aeromonas sobria

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activate enzyme Aeromonas sobria
Mg2+ activate enzyme Aeromonas sobria
Mn2+ activate enzyme Aeromonas sobria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Aeromonas sobria

Organism

Organism UniProt Comment Textmining
Aeromonas sobria
-
-
-
Aeromonas sobria AER14M
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aeromonas sobria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Aeromonas sobria AER14M (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Aeromonas sobria AER14M (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
biapenem + H2O
-
 Aeromonas sobria (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate
-
 ?
biapenem + H2O
-
 Aeromonas sobria AER14M (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate
-
 ?
carbenicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
carbenicillin + H2O
-
 Aeromonas sobria AER14M (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Aeromonas sobria (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Aeromonas sobria (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
additional information negligible hydrolysis of the cephalosporins cephaloridine, nitrocefin, cefoxitin, aztreonam Aeromonas sobria ?
-
 ?
additional information negligible hydrolysis of the cephalosporins cephaloridine, nitrocefin, cefoxitin, aztreonam Aeromonas sobria AER14M ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.133
-
 ampicillin
-
 Aeromonas sobria
0.433
-
 carbenicillin
-
 Aeromonas sobria
1.2
-
 benzylpenicillin
-
 Aeromonas sobria
1.5
-
 biapenem
-
 Aeromonas sobria
71
-
 Imipenem
-
 Aeromonas sobria
220
-
 meropenem
-
 Aeromonas sobria