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Literature summary for 3.5.2.6 extracted from
- Purification, characterization, and kinetic studies of a soluble Bacteroides fragilis metallo-beta-lactamase that provides multiple antibiotic resistance (1998), J. Biol. Chem., 273, 22402-22408.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacteroides fragilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Bacteroides fragilis |  |
| EDTA | - |
Bacteroides fragilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
nitrocefin | - |
Bacteroides fragilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Co2+ substituted enzyme: half of the activity of Zn2+enzyme | Bacteroides fragilis | |
| Zn2+ | required for activity | Bacteroides fragilis | |
| Zn2+ | two mol of Zn2+/mol protein | Bacteroides fragilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
- |
Bacteroides fragilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides fragilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteroides fragilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, purified enzyme, long time | Bacteroides fragilis |
| 4°C, purified enzyme, 1 week, no loss of activity | Bacteroides fragilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrocefin + H2O | - |
Bacteroides fragilis | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
ir | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 225 | - |
nitrocefin | - |
Bacteroides fragilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacteroides fragilis |
| 10 | - |
- |
Bacteroides fragilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | no activity below pH 5.5 | Bacteroides fragilis |
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