Literature summary for 3.5.2.5 extracted from

Conejero-Muriel, M.; Martinez-Gomez, A.I.; Martinez-Rodriguez, S.; Gavira, J.A.
Cloning, expression, purification, crystallization and preliminary X-ray characterization of allantoinase from Bacillus licheniformis ATCC 14580 (2014), Acta Crystallogr. Sect. F, 70, 1513-1516.

Search for more literature for 3.5.2.5

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Bacillus licheniformis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml 14 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 20% w/v PEG 3350, and 0.1 M potassium thiocyanate, pH 6.5, equilibration against 0.5 ml reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 3.5 A resolution	Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51163	-	x * 51163, sequence calculation	Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-allantoin + H2O	Bacillus licheniformis	allantoinase catalyzes the reversible hydrolysis of allantoin into allantoate by hydrolytic cleavage of the N1-C2 amide bond of the five-membered hydantoin ring. The enzyme shows an inverted enantioselectivity towards allantoin, R-enantioselective	allantoate	-	r
(S)-allantoin + H2O	Bacillus licheniformis ATCC 14580	allantoinase catalyzes the reversible hydrolysis of allantoin into allantoate by hydrolytic cleavage of the N1-C2 amide bond of the five-membered hydantoin ring. The enzyme shows an inverted enantioselectivity towards allantoin, R-enantioselective	allantoate	-	r

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	Q65LN0	-	-
Bacillus licheniformis ATCC 14580	Q65LN0	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, ultrafiltration, and dialysis	Bacillus licheniformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-allantoin + H2O	allantoinase catalyzes the reversible hydrolysis of allantoin into allantoate by hydrolytic cleavage of the N1-C2 amide bond of the five-membered hydantoin ring. The enzyme shows an inverted enantioselectivity towards allantoin, R-enantioselective	Bacillus licheniformis	allantoate	-	r
(S)-allantoin + H2O	allantoinase catalyzes the reversible hydrolysis of allantoin into allantoate by hydrolytic cleavage of the N1-C2 amide bond of the five-membered hydantoin ring. The enzyme shows an inverted enantioselectivity towards allantoin, R-enantioselective	Bacillus licheniformis ATCC 14580	allantoate	-	r

Subunits

Subunits	Comment	Organism
?	x * 51163, sequence calculation	Bacillus licheniformis

Synonyms

Synonyms	Comment	Organism
AllBali	-	Bacillus licheniformis

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Release 2023.1 (January 2023)